1994
A point mutation in Euglena gracilis chloroplast tRNA(Glu) uncouples protein and chlorophyll biosynthesis.
Stange-Thomann N, Thomann H, Lloyd A, Lyman H, Söll D. A point mutation in Euglena gracilis chloroplast tRNA(Glu) uncouples protein and chlorophyll biosynthesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 7947-7951. PMID: 8058739, PMCID: PMC44521, DOI: 10.1073/pnas.91.17.7947.Peer-Reviewed Original ResearchMeSH KeywordsAldehyde OxidoreductasesAnimalsBase SequenceBlotting, NorthernChlorophyllChloroplastsCloning, MolecularDNADNA PrimersEuglena gracilisIntramolecular TransferasesIsomerasesMolecular Sequence DataNucleic Acid ConformationPoint MutationPolymerase Chain ReactionProtein BiosynthesisRNA, Transfer, GluConceptsEuglena gracilis chloroplastsChlorophyll biosynthesisGlu-tRNA reductaseChlorophyll-deficient mutantsPoint mutationsChloroplast protein synthesisSequence-specific mannerDual-function moleculeC5 pathwayNADPH-dependent reductionSpecific cofactorsGluTRFirst enzymeGene productsUniversal precursorImportant identity elementAminomutase activitySequence analysisE. gracilisSecond enzymeTetrapyrrole pigmentsT-loopProtein synthesisBiosynthesisChloroplasts
1992
Organization and nucleotide sequence of the glutamine synthetase (glnA) gene from Lactobacillus delbrueckii subsp. bulgaricus
Ishino Y, Morgenthaler P, Hottinger H, Söll D. Organization and nucleotide sequence of the glutamine synthetase (glnA) gene from Lactobacillus delbrueckii subsp. bulgaricus. Applied And Environmental Microbiology 1992, 58: 3165-3169. PMID: 1359838, PMCID: PMC183065, DOI: 10.1128/aem.58.9.3165-3169.1992.Peer-Reviewed Original ResearchConceptsOpen reading frameGlnA geneReading frameGlnA gene expressionGlutamine synthetase geneL. delbrueckii subspDNA sequence analysisMaxicell methodLactobacillus delbrueckii subspDelbrueckii subspGlnR geneDeletion strainNegative eubacteriaPositive eubacteriaSynthetase geneExtensive homologyRepressor geneNucleotide sequencePresent upstreamGene expressionBamHI fragmentUnknown functionSequence analysisClostridium acetobutylicumGenesSwitching tRNA(Gln) identity from glutamine to tryptophan.
Rogers M, Adachi T, Inokuchi H, Söll D. Switching tRNA(Gln) identity from glutamine to tryptophan. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 3463-3467. PMID: 1565639, PMCID: PMC48888, DOI: 10.1073/pnas.89.8.3463.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acyl-tRNA SynthetasesAnticodonBase SequenceBeta-GalactosidaseCloning, MolecularEscherichia coliGenes, BacterialGenes, SuppressorGenes, SyntheticGlutamineMolecular Sequence DataMutagenesis, Site-DirectedNucleic Acid ConformationRNA, Transfer, GlnSuppression, GeneticTetrahydrofolate DehydrogenaseTryptophanConceptsOpal suppressorEscherichia coli glutaminyl-tRNA synthetaseAccuracy of aminoacylationGlutaminyl-tRNA synthetaseN-terminal sequence analysisEfficient suppressorYeast mitochondriaRespective tRNAsUCA anticodonAmber suppressorFol geneUGA codonUGA mutationsSequence analysisAlanine insertionAnticodonGenetic selectionBase pairsBase substitutionsSuppressorTRNATrpRSDihydrofolate reductasePosition 35Mutations
1991
delta-Aminolevulinic acid dehydratase deficiency can cause delta-aminolevulinate auxotrophy in Escherichia coli
O'Neill G, Thorbjarnardóttir S, Michelsen U, Pálsson S, Söll D, Eggertsson G. delta-Aminolevulinic acid dehydratase deficiency can cause delta-aminolevulinate auxotrophy in Escherichia coli. Journal Of Bacteriology 1991, 173: 94-100. PMID: 1987138, PMCID: PMC207161, DOI: 10.1128/jb.173.1.94-100.1991.Peer-Reviewed Original ResearchConceptsALA dehydratase activityEscherichia coliWild-type geneClasses of mutantsDNA sequence analysisAminoglycoside antibiotic kanamycinHeme biosynthetic pathwayALA biosynthesisWild-type DNAAuxotrophic phenotypeComplementation studiesDehydratase activityHemB geneBiosynthetic pathwayPositive regulationALA formationSame geneMutantsPenicillin enrichmentSequence analysisGenesAntibiotic kanamycinDiffusible productHemB mutantEnzymatic activity
1990
Introduction and Overview
Söll D. Introduction and Overview. Journal Of Chromatography Library 1990, 45: b1-b11. DOI: 10.1016/s0301-4770(08)61486-4.Peer-Reviewed Original ResearchDifferent amino acid specificitiesUse of UGAAmino acid specificityStandard genetic codeAmber suppressor tRNAVariety of organismsT7 RNA polymeraseGel sequencing methodsTRNA genesAmber mutantsGenetic codeRNA polymeraseSense codonsSuppressor tRNABiophysical instrumentationRelevant genesRNA fieldRNA sequencesSequence analysisDifferent suppressorsMolecular biologyCertain organismsTRNASequencing methodsGenes
1987
Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae
Weygand-Durasevic I, johnson-Burke D, Söll D. Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae. Nucleic Acids Research 1987, 15: 1887-1904. PMID: 3031581, PMCID: PMC340606, DOI: 10.1093/nar/15.5.1887.Peer-Reviewed Original ResearchConceptsSeryl-tRNA synthetaseSingle open reading frameAbundant yeast proteinsGenomic Southern blotsNuclease S1 analysisOpen reading frameTranslation initiation codonAmino acid sequenceKb SalI fragmentNucleotide sequence analysisAT-rich sequencesYeast proteinsStructural geneCodon usageS1 analysisTATA boxInitiation codonReading frameSer geneSalI fragmentAcid sequenceExpression librarySequence analysisRich sequencesSouthern blot
1986
The RNA required in the first step of chlorophyll biosynthesis is a chloroplast glutamate tRNA
Schön A, Krupp G, Gough S, Berry-Lowe S, Kannangara C, Söll D. The RNA required in the first step of chlorophyll biosynthesis is a chloroplast glutamate tRNA. Nature 1986, 322: 281-284. PMID: 3637637, DOI: 10.1038/322281a0.Peer-Reviewed Original ResearchConceptsΔ-aminolevulinatePeptide bond synthesisCognate amino acidMolecules of chlorophyllLow relative molecular massNucleotide sequence analysisRelative molecular massBond synthesisSubsequent reactionChlorophyll biosynthesisTransfer RNAUniversal precursorGlutamate tRNAAminoacyl bondSequence analysisNovel roleSerial affinity chromatographyMolecular massRNAAmino acidsComplete reactionBlue SepharoseAcceptor RNAReduction of glutamateReaction
1985
Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe.
Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D. Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe. Molecular And Cellular Biology 1985, 5: 808-815. PMID: 3921825, PMCID: PMC366785, DOI: 10.1128/mcb.5.4.808.Peer-Reviewed Original ResearchConceptsTRNA genesSchizosaccharomyces pombeGenomic clone bankEucaryotic tRNA genesTranscription control regionsIdentification of mutationsClone bankTRNA precursorsControl regionNonsense codonGenetic evidenceNonsense suppressorsRevertant allelesTranscriptional efficiencySaccharomyces cerevisiae extractSequence analysisSuppressor locusColony hybridizationMutational hotspotsPoint mutationsCerevisiae extractGenesPombeMutationsSplicingMutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe
Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D. Mutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe. Molecular And Cellular Biology 1985, 5: 808-815. DOI: 10.1128/mcb.5.4.808-815.1985.Peer-Reviewed Original ResearchTRNA genesGenomic clone bankEucaryotic tRNA genesTranscription control regionsIdentification of mutationsSchizosaccharomyces pombeClone bankTRNA precursorsControl regionNonsense codonGenetic evidenceNonsense suppressorsRevertant allelesTranscriptional efficiencySaccharomyces cerevisiae extractSequence analysisSuppressor locusColony hybridizationMutational hotspotsPoint mutationsCerevisiae extractGenesMutationsSup3SchizosaccharomycesMutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe
Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D. Mutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe. Molecular And Cellular Biology 1985, 5: 808-815. DOI: 10.1128/mcb.5.4.808-815.1985.Peer-Reviewed Original ResearchSup3-e geneTRNA genesInternal transcriptional control regionSuppression of nonsense codonsGenomic clone bankEucaryotic tRNA genesSaccharomyces cerevisiae extractsTranscriptional control regionIdentification of mutationsSchizosaccharomyces pombeColony hybridizationRevertant allelesTRNA precursorsClone bankNonsense codonSuppressor locusGenetic evidenceSequence analysisControl regionMutational hotspotsTranscription efficiencyPoint mutationsGenesMutationsSup3
1984
In vivo and in vitro transcription of the Escherichia coli glutaminyl-tRNA synthetase gene.
Cheung A, Söll D. In vivo and in vitro transcription of the Escherichia coli glutaminyl-tRNA synthetase gene. Journal Of Biological Chemistry 1984, 259: 9953-9958. PMID: 6086662, DOI: 10.1016/s0021-9258(17)42791-8.Peer-Reviewed Original ResearchConceptsGlutaminyl-tRNA synthetaseGlutaminyl-tRNA synthetase geneTranslation initiation codon AUGInitiation codon AUGPresence of tRNATermination codon UAAGlnS geneTerminator structureTranscription initiationSynthetase geneTranscription initiatesCodons UAARegulatory signalsCodon AUGTermination sitesTranscription productsSequence analysisStructure upstreamStructural regionsTranscriptionGenesTranscriptsSynthetaseMRNAGln
1982
Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA.
Cooley L, Appel B, Söll D. Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 6475-6479. PMID: 6292903, PMCID: PMC347149, DOI: 10.1073/pnas.79.21.6475.Peer-Reviewed Original ResearchConceptsMature tRNAHistidine tRNAPrimary transcriptHistidine tRNA genesGuanylate residuePost-transcriptional additionDrosophila Kc cellsTRNA genesDrosophila melanogasterSchizosaccharomyces pombeTRNAs resultsRNA speciesRNase PEukaryotic mRNAsKc cellsRNA precursorsTRNASequence analysisNucleotide additionAdditional nucleotidesPhosphodiester bondGenesNucleotidesMaturation schemeTranscripts
1980
Dimeric tRNA precursors in yeast
Schmidt O, Mao J, Ogden R, Beckmann J, Sakano H, Abelson J, Söll D. Dimeric tRNA precursors in yeast. Nature 1980, 287: 750-752. PMID: 6253814, DOI: 10.1038/287750a0.Peer-Reviewed Original ResearchConceptsDimeric tRNA precursorTRNA precursorsDNA fragmentsYeast tRNA genesDifferent genomic regionsNucleotide sequence analysisTRNA genesSaccharomyces cerevisiae1Xenopus nucleiGenomic regionsSpecific transcriptionTRNA speciesPrecursor RNARNA precursorsTRNA moleculesGene regionSequence analysisGene configurationEnzyme activityGenesMode of expressionFragmentsTranscriptionYeastTRNAAspDimeric transfer RNA precursors in S. pombe
Mao J, Schmidt O, Söll D. Dimeric transfer RNA precursors in S. pombe. Cell 1980, 21: 509-516. PMID: 7407924, DOI: 10.1016/0092-8674(80)90488-2.Peer-Reviewed Original ResearchConceptsTRNA genesNucleotide intronRNA precursorsDNA fragmentsNuclear extractsNucleotide spacerPrimary transcription productTransfer RNA precursorsNucleotide leader sequenceS. pombeTRNA sequencesTrailer sequencesRNA initiationLeader sequenceTRNA moleculesTranscription productsEnzymatic stepsSequence analysisRNA analysisXenopus oocytesGenesEndonuclease cleavageIntronsTerminusSequenceIN VITRO TRANSCRIPTION OF CLONED EUKARYOTIC tRNA GENES
Schmidt O, Hovemann B, Silverman S, Yamada H, Mao J, Söll D. IN VITRO TRANSCRIPTION OF CLONED EUKARYOTIC tRNA GENES. 1980, 179-188. DOI: 10.1016/b978-0-08-024417-4.50021-4.Peer-Reviewed Original ResearchTRNA genesTRNA precursorsEukaryotic tRNA genesYeast tRNA genesRegulatory DNA regionsRNA polymerase IIICorresponding RNA sequencesXenopus germinal vesicleXenopus nuclear extractsPrecursor tRNAsMature tRNADNA regionsPrimary transcriptTRNA biosynthesisPolymerase IIIGene transcriptionDNA sequencesNuclear extractsRNA sequencesSequence analysisTRNAGerminal vesicleGenesTranscriptionSequence