Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs.
Kohli J, Kwong T, Altruda F, Söll D, Wahl G. Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs. Journal Of Biological Chemistry 1979, 254: 1546-1551. PMID: 762155, DOI: 10.1016/s0021-9258(17)37806-7.Peer-Reviewed Original ResearchConceptsOpal suppressor tRNASuppressor tRNAReadthrough productSchizosaccharomyces pombeFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeOchre suppressor tRNAUGA termination codonBeta-globin mRNARabbit beta-globin mRNARabbit globin mRNAWheat germ extractSuppressor mutantsS. pombeNonsense suppressionPure tRNAsAlpha-globinSerine tRNATermination codonGlobin mRNATRNAPombeGerm extractSerineAssay systemIdentification and nucleotide sequence of the sup8-e UGA-suppressor leucine tRNA from Schizosaccharomyces pombe
Wetzel R, Kohli J, Altruda F, Söll D. Identification and nucleotide sequence of the sup8-e UGA-suppressor leucine tRNA from Schizosaccharomyces pombe. Molecular Genetics And Genomics 1979, 172: 221-228. PMID: 289895, DOI: 10.1007/bf00268286.Peer-Reviewed Original ResearchConceptsSuppressor tRNANucleotide sequenceTwo-dimensional gel electrophoresisS. pombe strainRabbit globin mRNAGel sequencing methodsWheat germ extractUGA suppressor tRNASchizosaccharomyces pombeAnticodon sequenceUGA codonOpal suppressionPombe strainGlobin mRNAC-AOHTRNALeucine tRNAGerm extractAnticodon loopSequencing methodsG-m5CGel electrophoresisSequenceSuppressor assaysSchizosaccharomyces