Partial purification of RNase P from Schizosaccharomyces pombe.
Kline L, Nishikawa S, Söll D. Partial purification of RNase P from Schizosaccharomyces pombe. Journal Of Biological Chemistry 1981, 256: 5058-5063. PMID: 6262315, DOI: 10.1016/s0021-9258(19)69366-x.Peer-Reviewed Original ResearchConceptsSimple assay procedureNucleic acid componentsColumn chromatographyReaction productsEnzyme purificationPhosphocellulose column chromatographyAcid componentsSynthetic substratesAssay procedurePurificationAcid solubilityPartial purificationSubstrateYeast RNase PSolubilityChromatographyProductsSeparationPhosphateTrichloroacetic acid solubility