1993
Identification of a 100‐kDa protein associated with nuclear ribonuclease P activity in Schizosaccharomyces pombe
ZIMMERLY S, DRAINAS D, SYLVERS L, Dieter S. Identification of a 100‐kDa protein associated with nuclear ribonuclease P activity in Schizosaccharomyces pombe. The FEBS Journal 1993, 217: 501-507. PMID: 8223594, DOI: 10.1111/j.1432-1033.1993.tb18270.x.Peer-Reviewed Original ResearchConceptsFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeGlycerol gradient fractionationCross-linking experimentsPrecursor tRNAsSchizosaccharomyces pombeRibonuclease PProtein interactsRNA componentProtein componentsP activityRibonuclease P activityApparent homogeneityDEAE-cellulose chromatographyPhosphocellulose chromatographySpecific fashionProtein
1990
Expression of the Synechocystis sp. strain PCC 6803 tRNA(Glu) gene provides tRNA for protein and chlorophyll biosynthesis
O'Neill G, Söll D. Expression of the Synechocystis sp. strain PCC 6803 tRNA(Glu) gene provides tRNA for protein and chlorophyll biosynthesis. Journal Of Bacteriology 1990, 172: 6363-6371. PMID: 2121711, PMCID: PMC526821, DOI: 10.1128/jb.172.11.6363-6371.1990.Peer-Reviewed Original ResearchConceptsSynechocystis 6803Synechocystis spFirst anticodon baseStrain PCC 6803Cyanobacterium Synechocystis spTotal tRNA populationAmount of chlorophyllNorthern blot analysisChlorophyll biosynthesisALA biosynthesisPrecursor tRNAsPCC 6803TRNA speciesProtein biosynthesisTRNA populationCellular RNAAminoacylation assaysChlorophyll levelsBiosynthesisAddition of inhibitorsBlot analysisTranslation systemDelta-aminolevulinic acidTRNAChlorophyll
1988
Yeast RNase P: catalytic activity and substrate binding are separate functions.
Nichols M, Söll D, Willis I. Yeast RNase P: catalytic activity and substrate binding are separate functions. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 1379-1383. PMID: 3278310, PMCID: PMC279774, DOI: 10.1073/pnas.85.5.1379.Peer-Reviewed Original ResearchConceptsPrecursor tRNAsRNase PSubstrate bindingGel retardationCatalytic functionRibonucleoprotein RNase PDistinct sequence preferencesEnzyme catalytic functionRNase P cleavage siteMature tRNARNase P.Catalytic integrityTRNA precursorsRNA moietyRNA componentSequence preferenceTRNATRNA complexProtein componentsAcceptor stemEnzyme mechanismMaximal cleavageSecond nucleotideCleavage siteEnzyme
1980
Two control regions for eukaryotic tRNA gene transcription.
DeFranco D, Schmidt O, Söll D. Two control regions for eukaryotic tRNA gene transcription. Proceedings Of The National Academy Of Sciences Of The United States Of America 1980, 77: 3365-3368. PMID: 6774336, PMCID: PMC349616, DOI: 10.1073/pnas.77.6.3365.Peer-Reviewed Original ResearchConceptsControl regionTRNALys geneGene transcriptionEukaryotic tRNA gene transcriptionTRNA gene transcriptionInternal control regionIdentical coding sequenceShort leader sequencePrecursor tRNAsTranscription initiationMature tRNALeader sequenceCoding sequencePBR322 sequencesNuclear extractsRecombinant plasmidTranscriptionXenopus oocytesGenesTRNASequenceIN VITRO TRANSCRIPTION OF CLONED EUKARYOTIC tRNA GENES
Schmidt O, Hovemann B, Silverman S, Yamada H, Mao J, Söll D. IN VITRO TRANSCRIPTION OF CLONED EUKARYOTIC tRNA GENES. 1980, 179-188. DOI: 10.1016/b978-0-08-024417-4.50021-4.Peer-Reviewed Original ResearchTRNA genesTRNA precursorsEukaryotic tRNA genesYeast tRNA genesRegulatory DNA regionsRNA polymerase IIICorresponding RNA sequencesXenopus germinal vesicleXenopus nuclear extractsPrecursor tRNAsMature tRNADNA regionsPrimary transcriptTRNA biosynthesisPolymerase IIIGene transcriptionDNA sequencesNuclear extractsRNA sequencesSequence analysisTRNAGerminal vesicleGenesTranscriptionSequence
1978
Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.
Schmidt O, Mao J, Silverman S, Hovemann B, Söll D. Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts. Proceedings Of The National Academy Of Sciences Of The United States Of America 1978, 75: 4819-4823. PMID: 105357, PMCID: PMC336212, DOI: 10.1073/pnas.75.10.4819.Peer-Reviewed Original ResearchConceptsGerminal vesicle extractsTRNA genesRNA speciesVesicle extractsEukaryotic gene transcriptionEukaryotic tRNA genesRegulatory DNA regionsPrecursor tRNA moleculesXenopus germinal vesiclePrecursor tRNAsSpecific transcriptionDNA regionsTRNA biosynthesisTRNA precursorsNucleotide modificationsGene transcriptionTRNA moleculesTranscription systemNucleotide analysisTRNAGerminal vesicleGenesTranscriptionTRNALysSpecies
1971
Enzymatic Modification of Transfer RNA
Söll D. Enzymatic Modification of Transfer RNA. Science 1971, 173: 293-299. PMID: 4934576, DOI: 10.1126/science.173.3994.293.Peer-Reviewed Original ResearchConceptsTRNA-modifying enzymesTRNA moleculesTRNA genesTransfer RNAAmino acid biosynthesisParticular tRNA speciesNucleic acid-protein interactionsAminoacyl-tRNA synthetaseSimple selection procedureAmino acid acceptorPrecursor tRNAsActive tRNAMature tRNACellular processesTRNA speciesRegulatory mutantsAcid biosynthesisRNA precursorsProtein factorsIsoacceptor tRNAsDifferent amino acidsNucleotide sequencePolynucleotide levelBiological functionsTRNA