2002
A one‐step method for in vitro production of tRNA transcripts
Korencić D, Söll D, Ambrogelly A. A one‐step method for in vitro production of tRNA transcripts. Nucleic Acids Research 2002, 30: e105-e105. PMID: 12384607, PMCID: PMC137149, DOI: 10.1093/nar/gnf104.Peer-Reviewed Original ResearchConceptsTRNA transcriptsT7 RNA polymeraseLarge-scale plasmid preparationTRNA genesMicrobial genomesTRNA functionsDNA promoterRNA polymeraseRNA moleculesT7 promoterBiochemical characterizationTranscription templateDNA templateNew enzymeTranscriptsLarge oligonucleotidesTranscriptionGood substratePromoterShort oligonucleotidesEnzymatic digestionRapid productionPlasmid preparationsGenomeOligonucleotide
1981
Identification of regulatory sequences contained in the 5'-flanking region of Drosophila lysine tRNA2 genes.
DeFranco D, Sharp S, Söll D. Identification of regulatory sequences contained in the 5'-flanking region of Drosophila lysine tRNA2 genes. Journal Of Biological Chemistry 1981, 256: 12424-12429. PMID: 6913581, DOI: 10.1016/s0021-9258(18)43290-5.Peer-Reviewed Original ResearchConceptsTRNA genesTranscriptional repressionMature tRNATemplate activityDrosophila tRNA genesLow template activityInsertion of nucleotidesPoor transcriptional activityRegulatory sequencesDeletion analysisNucleotides 23Transcriptional activityAdditional nucleotidesGenesTRNARepressionNucleotidesTranscriptionComplete lossSequenceDeletionOligonucleotideOligonucleotide sequencesRegionActivity