1991
Two glutamyl-tRNA reductase activities in Escherichia coli
Jahn D, Michelsen U, Söll D. Two glutamyl-tRNA reductase activities in Escherichia coli. Journal Of Biological Chemistry 1991, 266: 2542-2548. PMID: 1990004, DOI: 10.1016/s0021-9258(18)52279-1.Peer-Reviewed Original ResearchConceptsReductase activityGlu-tRNA reductaseMolecular massEscherichia coliApparent molecular massDifferent chromatographic separationsSequence-specific recognitionGlycerol gradient centrifugationThree-step conversionTetrapyrrole biosynthesisChlamydomonas reinhardtiiE. coli K12ALA formationChromatographic separationKey enzymeMonomeric structureActive enzymeBacillus subtilisColi K12Nondenaturing conditionsHomogeneous proteinMolecular weightDelta-aminolevulinic acidEnzyme activityAddition of GTP
1990
Enzymatic addition of guanylate to histidine transfer RNA
Williams J, Cooley L, Söll D. Enzymatic addition of guanylate to histidine transfer RNA. Methods In Enzymology 1990, 181: 451-462. PMID: 2166216, DOI: 10.1016/0076-6879(90)81143-i.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineChromatography, AffinityChromatography, DEAE-CelluloseChromatography, Ion ExchangeDrosophilaElectrophoresis, Polyacrylamide GelGuanosine TriphosphateKineticsNucleotidyltransferasesPhosphorus RadioisotopesRadioisotope Dilution TechniqueRNA, Transfer, Amino Acid-SpecificRNA, Transfer, HisSaccharomyces cerevisiaeSubstrate SpecificityConceptsHistidine tRNATransfer RNABacteriophage T5Yeast enzymeEnzyme migratesUridine residuesExtra nucleotidesLigase mechanismAdditional nucleotidesEnzymatic additionGel filtration chromatographyEnzyme intermediateTRNAAbsolute requirementEnzymeMolecular weightNucleotidesUltrogel AcA 34Filtration chromatographyATPDrosophilaAcA 34Molecular weight markersYeastTitration experiments
1985
A wheat HMW glutenin subunit gene reveals a highly repeated structure
Sugiyama T, Rafalski A, Peterson D, Söll D. A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Research 1985, 13: 8729-8737. PMID: 3001648, PMCID: PMC318947, DOI: 10.1093/nar/13.24.8729.Peer-Reviewed Original ResearchConceptsTypical eukaryotic promoterWheat genomic libraryHMW glutenin subunit genesRNA initiation siteGlutenin subunit genesPartial cDNA cloneAmino acid sequenceMature proteinGenomic libraryGlutenin genesEukaryotic promotersCDNA clonesGene sequencesProline contentSubunit geneAcid sequenceGrain endospermInitiation siteQuality of wheatGenesSynthetic oligonucleotidesHigh glutamineMolecular weightProteinClones
1972
N6 - (Δ2 - Isopentenyl) Adenosine: Biosynthesis in vitro in transfer RNA by an enzyme purified from Eschericha coli
Bartz J, Söll D. N6 - (Δ2 - Isopentenyl) Adenosine: Biosynthesis in vitro in transfer RNA by an enzyme purified from Eschericha coli. Biochimie 1972, 54: 31-39. PMID: 4346747, DOI: 10.1016/s0300-9084(72)80035-x.Peer-Reviewed Original Research
1970
N6-(Δ2-isopentenyl)adenosine: Biosynthesis in vitro in transfer RNA by an enzyme purified from escherichia coli
Bartz J, Kline L, Söll D. N6-(Δ2-isopentenyl)adenosine: Biosynthesis in vitro in transfer RNA by an enzyme purified from escherichia coli. Biochemical And Biophysical Research Communications 1970, 40: 1481-1487. PMID: 4326583, DOI: 10.1016/0006-291x(70)90035-5.Peer-Reviewed Original ResearchMeSH KeywordsAdenineAdenine NucleotidesAlkenesAnimalsCentrifugation, Density GradientChromatography, PaperColiphagesEscherichia coliHydrogen-Ion ConcentrationHydrolysisLiverMolecular WeightMycoplasmaNucleosidesOrganophosphorus CompoundsPhosphoric AcidsPolynucleotidesPotassium PermanganateRatsRNA, BacterialRNA, TransferRNA, ViralTransferasesPurification of Leucyl Transfer Ribonucleic Acid Synthetase from Escherichia coli
Hayashi H, Knowles J, Katze J, Lapointe J, Söll D. Purification of Leucyl Transfer Ribonucleic Acid Synthetase from Escherichia coli. Journal Of Biological Chemistry 1970, 245: 1401-1406. PMID: 4986473, DOI: 10.1016/s0021-9258(18)63250-8.Peer-Reviewed Original ResearchMeSH KeywordsAlkylationAmino AcidsChromatographyChromatography, DEAE-CelluloseDrug StabilityEdetic AcidElectrophoresisEscherichia coliHydrogen-Ion ConcentrationHydroxyapatitesLeucineLigasesMethodsMolecular WeightOxidation-ReductionRNA, BacterialRNA, TransferSodium ChlorideTemperatureTime FactorsUltracentrifugationConceptsEscherichia coliSynthetaseColiSedimentation coefficientEnzymePresence of EDTAMolecular weightPurification