1985
Transcription of a Drosophila tRNAArg gene in yeast extract: 5′-flanking sequence dependence for transcription in a heterologous system
Schaack J, Söll D. Transcription of a Drosophila tRNAArg gene in yeast extract: 5′-flanking sequence dependence for transcription in a heterologous system. Nucleic Acids Research 1985, 13: 2803-2814. PMID: 3889849, PMCID: PMC341195, DOI: 10.1093/nar/13.8.2803.Peer-Reviewed Original ResearchConceptsDrosophila Kc cell extractS. cerevisiae extractTRNA genesCerevisiae extractDrosophila tRNAArg geneCell extractsDrosophila tRNA genesDrosophila Kc cellsTRNAArg geneTranscription extractActive transcriptionTranscription kineticsExtracts of SaccharomycesSteady-state levelsKc cellsHeterologous systemsS. cerevisiaeSequence requirementsLag phaseTranscriptionGenesHeterologous combinationsPARGPosition 21Deletion
1983
Partial purification of Drosophila Kc cell RNA polymerase III transcription components. Evidence for shared 5 S RNA and tRNA gene factors.
Burke D, Schaack J, Sharp S, Söll D. Partial purification of Drosophila Kc cell RNA polymerase III transcription components. Evidence for shared 5 S RNA and tRNA gene factors. Journal Of Biological Chemistry 1983, 258: 15224-15231. PMID: 6197413, DOI: 10.1016/s0021-9258(17)43797-5.Peer-Reviewed Original ResearchConceptsS RNA geneTranscription componentsRNA polymerase IIIRNA genesPolymerase IIIStable transcription complex formationReconstitution of transcriptionTranscription complex formationDrosophila Kc cellsCM-Sepharose column chromatographyDrosophila tRNAKc cellsTranscription factorsS RNATRNAGene factorsCompetition experimentsTranscriptionGenesComplex formationPartial purificationDEAE-SephadexReconstitutionCofractionationColumn chromatography
1982
Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA.
Cooley L, Appel B, Söll D. Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 6475-6479. PMID: 6292903, PMCID: PMC347149, DOI: 10.1073/pnas.79.21.6475.Peer-Reviewed Original ResearchConceptsMature tRNAHistidine tRNAPrimary transcriptHistidine tRNA genesGuanylate residuePost-transcriptional additionDrosophila Kc cellsTRNA genesDrosophila melanogasterSchizosaccharomyces pombeTRNAs resultsRNA speciesRNase PEukaryotic mRNAsKc cellsRNA precursorsTRNASequence analysisNucleotide additionAdditional nucleotidesPhosphodiester bondGenesNucleotidesMaturation schemeTranscriptsTHE IN VITRO TRANSCRIPTION OF DROSOPHILA tRNA GENES
Dingermann T, Sharp S, Schaack J, DeFranco D, Johnson D, Cooley L, Söll D. THE IN VITRO TRANSCRIPTION OF DROSOPHILA tRNA GENES. 1982, 219-234. DOI: 10.1016/b978-0-12-525960-6.50021-3.Peer-Reviewed Original ResearchT-control regionDrosophila tRNA genesTRNA genesTranscription initiationGene-specific transcription factorsD-control regionMutant tRNA genesDrosophila Kc cellsSpecific transcription factorsMature tRNATranscription complexTermination signalKc cellsPrimary transcriptTranscription factorsControl regionFactor bindingGene transcriptionLeader sequenceFactor bindsPurine triphosphatesRich stretchEfficient terminationD-loopFlanking sequences
1981
Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract
Dingermann T, Sharp S, Appel B, DeFranco D, Mount S, Heiermann R, Pongs O, Söll D. Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract. Nucleic Acids Research 1981, 9: 3907-3918. PMID: 6170932, PMCID: PMC327404, DOI: 10.1093/nar/9.16.3907.Peer-Reviewed Original Research