1988
Escherichia coli glutaminyl-tRNA synthetase: a single amino acid replacement relaxes rRNA specificity.
Uemura H, Conley J, Yamao F, Rogers J, Söll D. Escherichia coli glutaminyl-tRNA synthetase: a single amino acid replacement relaxes rRNA specificity. Protein Sequences And Data Analysis 1988, 1: 479-85. PMID: 2464170.Peer-Reviewed Original ResearchConceptsGlutaminyl-tRNA synthetaseTRNA bindingEscherichia coli glutaminyl-tRNA synthetaseExtensive homology searchesSingle amino acid replacementSingle amino acid changeRegion of homologyAminoacyl-tRNA synthetasesAmino acid replacementsAminoacyl adenylate formationAmino acids 235Amino acid changesLittle apparent similarityGlnS geneTRNA discriminationHomology searchGene productsAcid replacementsShare regionsDifferent tRNAsShort stretchesGenetic selectionAcid changesAsn changeHomology
1984
Transfer RNA mischarging mediated by a mutant Escherichia coli glutaminyl-tRNA synthetase.
Inokuchi H, Hoben P, Yamao F, Ozeki H, Söll D. Transfer RNA mischarging mediated by a mutant Escherichia coli glutaminyl-tRNA synthetase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 5076-5080. PMID: 6382258, PMCID: PMC391640, DOI: 10.1073/pnas.81.16.5076.Peer-Reviewed Original ResearchConceptsGlutaminyl-tRNA synthetaseGlnS geneEscherichia coli glutaminyl-tRNA synthetaseAminoacyl-tRNA synthetase genesEarlier genetic studiesAmber suppressor tRNAWild-type enzymeSynthetase geneTRNA speciesAmber anticodonAmber mutationMutant tRNAsSuppressor tRNAGene productsAltered specificityGln mutantMutant geneTransducing phageEnzyme structureGenetic studiesTRNAGenesMischargingBiochemical meansAminoacylation reactionIn vivo and in vitro transcription of the Escherichia coli glutaminyl-tRNA synthetase gene.
Cheung A, Söll D. In vivo and in vitro transcription of the Escherichia coli glutaminyl-tRNA synthetase gene. Journal Of Biological Chemistry 1984, 259: 9953-9958. PMID: 6086662, DOI: 10.1016/s0021-9258(17)42791-8.Peer-Reviewed Original ResearchConceptsGlutaminyl-tRNA synthetaseGlutaminyl-tRNA synthetase geneTranslation initiation codon AUGInitiation codon AUGPresence of tRNATermination codon UAAGlnS geneTerminator structureTranscription initiationSynthetase geneTranscription initiatesCodons UAARegulatory signalsCodon AUGTermination sitesTranscription productsSequence analysisStructure upstreamStructural regionsTranscriptionGenesTranscriptsSynthetaseMRNAGln
1982
Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene.
Yamao F, Inokuchi H, Cheung A, Ozeki H, Söll D. Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene. Journal Of Biological Chemistry 1982, 257: 11639-11643. PMID: 6288695, DOI: 10.1016/s0021-9258(18)33810-9.Peer-Reviewed Original Research