Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs.
Kohli J, Kwong T, Altruda F, Söll D, Wahl G. Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs. Journal Of Biological Chemistry 1979, 254: 1546-1551. PMID: 762155, DOI: 10.1016/s0021-9258(17)37806-7.Peer-Reviewed Original ResearchConceptsOpal suppressor tRNASuppressor tRNAReadthrough productSchizosaccharomyces pombeFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeOchre suppressor tRNAUGA termination codonBeta-globin mRNARabbit beta-globin mRNARabbit globin mRNAWheat germ extractSuppressor mutantsS. pombeNonsense suppressionPure tRNAsAlpha-globinSerine tRNATermination codonGlobin mRNATRNAPombeGerm extractSerineAssay system