1995
Selective Coupling of β2-Adrenergic Receptor to Hematopoietic-specific G Proteins (∗)
Wu D, Kuang Y, Wu Y, Jiang H. Selective Coupling of β2-Adrenergic Receptor to Hematopoietic-specific G Proteins (∗). Journal Of Biological Chemistry 1995, 270: 16008-16010. PMID: 7608160, DOI: 10.1074/jbc.270.27.16008.Peer-Reviewed Original ResearchConceptsG alpha 16G alpha 15G alpha 11G alpha qG alpha 14Alpha 14Alpha 11Alpha qPhospholipase CAlpha 15G beta gammaAR agonist isoprenalineAlpha 16Norepinephrine-induced increaseNorepinephrine-induced activationG proteinsActivation of PLCCOS-7 cellsReceptor-mediated activationAccumulation of inositolConcentration-dependent mannerAntagonist propranololCotransfected cellsΒ2-adrenergic receptorAgonist isoprenaline
1993
Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins.
Wu D, Jiang H, Katz A, Simon M. Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins. Journal Of Biological Chemistry 1993, 268: 3704-3709. PMID: 8381437, DOI: 10.1016/s0021-9258(18)53750-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarbacholCell LineCloning, MolecularEnzyme ActivationGTP-Binding ProteinsInositol PhosphatesKineticsMolecular Sequence DataMutagenesisNorepinephrinePhosphatidylinositol Diacylglycerol-LyasePhosphoric Diester HydrolasesPlasmidsReceptors, MuscarinicRecombinant ProteinsRestriction MappingSequence DeletionTransfectionConceptsG alpha q.PLC-beta 1COS-7 cellsG alpha qAlpha q.Alpha subunitG protein alpha subunitsAlpha qMuscarinic acetylcholine receptor type 1G alpha subunitsPhosphoinositide-specific phospholipase CIntrinsic enzymatic activitySpecific deletionAmino acid sequenceLigand-induced releasePhospholipase C beta 1C beta 1CDNA clonesAcid sequenceC-terminusG proteinsTerminal endDependent activationPhospholipase CAlpha 1C-adrenergic receptor
1992
Activation of phospholipase C by alpha 1-adrenergic receptors is mediated by the alpha subunits of Gq family.
Wu D, Katz A, Lee C, Simon M. Activation of phospholipase C by alpha 1-adrenergic receptors is mediated by the alpha subunits of Gq family. Journal Of Biological Chemistry 1992, 267: 25798-25802. PMID: 1334487, DOI: 10.1016/s0021-9258(18)35680-1.Peer-Reviewed Original ResearchConceptsG alpha 11G alpha 16G alpha qG alpha 14Alpha 14Phospholipase C beta 1C beta 1Alpha 11Alpha qBeta 1C receptorAlpha 16Cos-7 cellsPhospholipase CAlpha 1Subtypes of alphaRegulatory protein alpha subunitFunctional couplingG alpha subunitsAlpha subunitInositol phosphate releaseG alpha q/G alpha 11Synergistic responseReceptor subtypesSame assay system