Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C
Katz A, Wu D, Simon M. Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C. Nature 1992, 360: 686-689. PMID: 1465134, DOI: 10.1038/360686a0.Peer-Reviewed Original ResearchConceptsΑ-subunitG proteinsExchange of GDPVitro reconstitution experimentsReceptor-mediated releaseDifferent G proteinsFree GβγPhospholipase C activationGβγ complexG proteins resultsGα subunitsHL-60 cellsGβγ subunitsTransfection assaysPertussis toxinΓ subunitProtein resultsGβγReconstitution experimentsDifferent isoformsSpecific receptorsΒ2 isoformPhospholipase CΒ1 isoformsSubunitsActivation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells.
Wu D, Lee C, Rhee S, Simon M. Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells. Journal Of Biological Chemistry 1992, 267: 1811-1817. PMID: 1309799, DOI: 10.1016/s0021-9258(18)46018-8.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesCell LineCell MembraneEnzyme ActivationGTP-Binding ProteinsGuanosine 5'-O-(3-Thiotriphosphate)InositolInositol PhosphatesIsoenzymesKineticsMacromolecular SubstancesMolecular Sequence DataMutagenesis, Site-DirectedPeptidesPhosphatidylinositol Diacylglycerol-LyasePhosphoinositide Phospholipase CPhosphoric Diester HydrolasesTransfectionType C PhospholipasesConceptsG alpha 11G alpha qCos-7 cellsG alpha zAlpha qPI-PLCAlpha 11Alpha subunitAlpha zG protein alpha subunitsProtein alpha subunitsAlpha-subunit cDNAHigh steady-state levelsPhospholipase C beta 1Introduction of mutationsCell-free systemHigh-efficiency transient transfectionG protein activationC beta 1Glutamine 205Steady-state levelsBeta 1Transient transfectionProtein activationLeucine mutant