1998
Guanine nucleotide exchange factor GEF115 specifically mediates activation of Rho and serum response factor by the G protein α subunit Gα13
Mao J, Yuan H, Xie W, Wu D. Guanine nucleotide exchange factor GEF115 specifically mediates activation of Rho and serum response factor by the G protein α subunit Gα13. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 12973-12976. PMID: 9789025, PMCID: PMC23675, DOI: 10.1073/pnas.95.22.12973.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SequenceAnimalsCOS CellsDNA-Binding ProteinsGene Expression RegulationGTPase-Activating ProteinsGTP-Binding ProteinsGuanine Nucleotide Exchange FactorsKineticsLuciferasesMiceMolecular Sequence DataNuclear ProteinsProteinsRecombinant Fusion ProteinsRhoA GTP-Binding ProteinSequence AlignmentSequence Homology, Amino AcidSerum Response FactorSignal TransductionTranscription FactorsTranscription, GeneticTransfectionConceptsSerum response factorActivation of RhoSRF activationHeterotrimeric G protein alpha subunitsG protein signaling (RGS) domainG protein alpha subunitsTerminal partRho-specific guanineProtein alpha subunitsResponse factorDomain deletion mutantSignal transduction pathwaysNIH 3T3 cellsDbl homologyRGS activitySequence motifsTransduction pathwaysGalpha13Signaling domainsGalpha12Alpha subunitMutantsSynergistic activationGalphaqTransfection system
1997
Two Basic Amino Acids in the Second Inner Loop of the Interleukin-8 Receptor Are Essential for Gα16 Coupling*
Xie W, Jiang H, Wu Y, Wu D. Two Basic Amino Acids in the Second Inner Loop of the Interleukin-8 Receptor Are Essential for Gα16 Coupling*. Journal Of Biological Chemistry 1997, 272: 24948-24951. PMID: 9312098, DOI: 10.1074/jbc.272.40.24948.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDArginineBinding SitesCOS CellsGTP-Binding ProteinsInterleukin-8KineticsLysineMolecular Sequence DataMutagenesis, Site-DirectedPhosphatidylinositol Diacylglycerol-LyaseProtein Structure, SecondaryReceptors, InterleukinReceptors, Interleukin-8ARecombinant Fusion ProteinsTransfectionType C Phospholipases
1996
Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma.
Kuang Y, Wu Y, Smrcka A, Jiang H, Wu D. Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 2964-2968. PMID: 8610151, PMCID: PMC39743, DOI: 10.1073/pnas.93.7.2964.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDBlotting, WesternCell LineChlorocebus aethiopsDNA, ComplementaryGlutamic AcidGTP-Binding ProteinsHumansIsoenzymesKidneyMacromolecular SubstancesMammalsMutagenesis, Site-DirectedPoint MutationPolymerase Chain ReactionReceptor, Anaphylatoxin C5aReceptors, ComplementRecombinant Fusion ProteinsRecombinant ProteinsTransfectionType C PhospholipasesConceptsCOS-7 cellsGlutathione S-transferaseGbeta gammaPLC-beta2Fusion proteinBeta gamma subunitsLigand-induced activationAlpha1B-adrenergic receptorMammalian expression plasmidX boxG proteinsY boxExpression plasmidAmino acidsS-transferaseProteinSequenceResiduesCellsActivationBeta2GalphaGbetaC5a receptorCDNA