1997
Two Basic Amino Acids in the Second Inner Loop of the Interleukin-8 Receptor Are Essential for Gα16 Coupling*
Xie W, Jiang H, Wu Y, Wu D. Two Basic Amino Acids in the Second Inner Loop of the Interleukin-8 Receptor Are Essential for Gα16 Coupling*. Journal Of Biological Chemistry 1997, 272: 24948-24951. PMID: 9312098, DOI: 10.1074/jbc.272.40.24948.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDArginineBinding SitesCOS CellsGTP-Binding ProteinsInterleukin-8KineticsLysineMolecular Sequence DataMutagenesis, Site-DirectedPhosphatidylinositol Diacylglycerol-LyaseProtein Structure, SecondaryReceptors, InterleukinReceptors, Interleukin-8ARecombinant Fusion ProteinsTransfectionType C Phospholipases
1996
Pertussis Toxin-sensitive Activation of Phospholipase C by the C5a and fMet-Leu-Phe Receptors*
Jiang H, Kuang Y, Wu Y, Smrcka A, Simon M, Wu D. Pertussis Toxin-sensitive Activation of Phospholipase C by the C5a and fMet-Leu-Phe Receptors*. Journal Of Biological Chemistry 1996, 271: 13430-13434. PMID: 8662841, DOI: 10.1074/jbc.271.23.13430.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDCell LineEnzyme ActivationGTP-Binding ProteinsMolecular Sequence DataPertussis ToxinReceptor, Anaphylatoxin C5aReceptors, ComplementReceptors, Formyl PeptideReceptors, ImmunologicReceptors, PeptideSignal TransductionTransfectionType C PhospholipasesVirulence Factors, BordetellaConceptsPLC-beta3PTX-sensitive G proteinsG proteinsFMet-LeuGbetagamma subunitsCOS-7 cellsPhospholipase CPLC-beta2Receptor-mediated PLC activationPTX-sensitive mannerFMLP-mediated activationPertussis toxinPhe receptorCell activationGi proteinsChemoattractant receptorsReceptorsPLC activationC5aBeta2Gq classActivationBeta3Betagamma subunitsSignal transduction pathways
1992
Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C
Katz A, Wu D, Simon M. Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C. Nature 1992, 360: 686-689. PMID: 1465134, DOI: 10.1038/360686a0.Peer-Reviewed Original ResearchConceptsΑ-subunitG proteinsExchange of GDPVitro reconstitution experimentsReceptor-mediated releaseDifferent G proteinsFree GβγPhospholipase C activationGβγ complexG proteins resultsGα subunitsHL-60 cellsGβγ subunitsTransfection assaysPertussis toxinΓ subunitProtein resultsGβγReconstitution experimentsDifferent isoformsSpecific receptorsΒ2 isoformPhospholipase CΒ1 isoformsSubunits