2017
Local Delivery of the Toll-Like Receptor 9 Ligand CpG Downregulates Host Immune and Inflammatory Responses, Ameliorating Established Leishmania (Viannia) panamensis Chronic Infection
Ehrlich AK, Fernández OL, Rodriguez-Pinto D, Castilho TM, Caridad M, Goldsmith-Pestana K, Saravia NG, McMahon-Pratt D. Local Delivery of the Toll-Like Receptor 9 Ligand CpG Downregulates Host Immune and Inflammatory Responses, Ameliorating Established Leishmania (Viannia) panamensis Chronic Infection. Infection And Immunity 2017, 85: 10.1128/iai.00981-16. PMID: 28052994, PMCID: PMC5328479, DOI: 10.1128/iai.00981-16.Peer-Reviewed Original ResearchConceptsAntigen-presenting cellsPeripheral blood mononuclear cellsCutaneous leishmaniasisB cellsIL-17IL-13Inflammatory responseMouse modelToll-like receptor 9 ligand CpGAlternate therapeutic approachCurrent treatment optionsBlood mononuclear cellsMixed inflammatory responseRegulatory cell functionProduction of IFNPredominant etiologic agentDose-response effectHost immune responseCell populationsGrowth factor βCpG treatmentRegulatory cellsChemokine responsesIL-10Host Immune
2014
Chronicity of Dermal Leishmaniasis Caused by Leishmania panamensis Is Associated with Parasite-Mediated Induction of Chemokine Gene Expression
Navas A, Vargas DA, Freudzon M, McMahon-Pratt D, Saravia NG, Gómez MA. Chronicity of Dermal Leishmaniasis Caused by Leishmania panamensis Is Associated with Parasite-Mediated Induction of Chemokine Gene Expression. Infection And Immunity 2014, 82: 2872-2880. PMID: 24752514, PMCID: PMC4097649, DOI: 10.1128/iai.01133-13.Peer-Reviewed Original ResearchConceptsSelf-healed patientsChronic cutaneous leishmaniasisDermal leishmaniasisChemokine gene expressionClinical outcomesBiopsy specimensChronic diseasesInflammatory responseLeishmania panamensisExpression levelsCCR5 receptor geneInduction of CXCL5Montenegro skin testLesion biopsy specimensL. panamensisSelf-healing diseaseNew therapeutic targetsReverse transcription-quantitative PCRTranscription-quantitative PCRChemotactic chemokinesInflammation contributesSkin testAsymptomatic infectionInflammatory activationImmune cellsCD4 T cell activation by B cells in human Leishmania (Viannia)infection
Rodriguez-Pinto D, Saravia NG, McMahon-Pratt D. CD4 T cell activation by B cells in human Leishmania (Viannia)infection. BMC Infectious Diseases 2014, 14: 108. PMID: 24568275, PMCID: PMC3937821, DOI: 10.1186/1471-2334-14-108.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBiotinB-LymphocytesCD4-Positive T-LymphocytesColombiaFemaleFlow CytometryFluorescein-5-isothiocyanateGene Expression RegulationHumansImmunoglobulin MInterferon-gammaInterleukin-6Leishmania braziliensisLeishmaniasis, CutaneousLeukocytes, MononuclearLymphocyte ActivationMaleMiddle AgedOvalbuminTumor Necrosis Factor-alphaYoung AdultConceptsCD4 T cellsCutaneous leishmaniasis patientsT cellsB cellsLeishmaniasis patientsT cell activationLeishmania antigenImmune responseCell activationT-cell activation parametersSpecific CD4 T cellsEffective adaptive immune responseCD4 T cell activationB-cell activation markersCultures of PBMCUpregulation of CD86Cell activation markersCostimulatory molecule CD86Activation markers CD25Adaptive immune responsesHuman cutaneous leishmaniasisPurified B cellsT cell culturesHuman B cell linesHuman B cells
2010
Murine model of chronic L. (Viannia) panamensis infection: Role of IL‐13 in disease
Castilho TM, Goldsmith‐Pestana K, Lozano C, Valderrama L, Saravia NG, McMahon‐Pratt D. Murine model of chronic L. (Viannia) panamensis infection: Role of IL‐13 in disease. European Journal Of Immunology 2010, 40: 2816-2829. PMID: 20827674, PMCID: PMC3289133, DOI: 10.1002/eji.201040384.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAnimalsChronic DiseaseDisease Models, AnimalEnzyme-Linked Immunosorbent AssayFemaleHumansInterferon-gammaInterleukin-13LeishmaniaLeishmaniasis, CutaneousMaleMiceMice, Inbred BALB CMice, KnockoutMiddle AgedReceptors, Interleukin-4Th1 CellsTh2 CellsTumor Necrosis Factor-alphaYoung AdultConceptsL. panamensis infectionsIL-13Panamensis infectionChronic diseasesImmunodeficient miceMurine modelMixed Th1/Th2 responseBALB/c mouse modelTh1/Th2 responsePrevalent etiologic agentHuman cutaneous leishmaniasisPresence of TNFPrevention of leishmaniasisIL-17Immunological mechanismsTh2 responsesIL-10Recurrent lesionsChronic infectionEvident lesionsMice resemblesT cellsImmune responsePersistent infectionLeishmania organisms
1992
Cutaneous Leishmaniasis: Review of 59 Cases Seen at the National Institutes of Health
Melby P, Kreutzer R, McMahon-Pratt D, Gam A, Neva F. Cutaneous Leishmaniasis: Review of 59 Cases Seen at the National Institutes of Health. Clinical Infectious Diseases 1992, 15: 924-937. PMID: 1457663, DOI: 10.1093/clind/15.6.924.Peer-Reviewed Original ResearchConceptsCutaneous leishmaniasisEndemic areasCutaneous diseaseGroup of patientsDiffuse cutaneous leishmaniasisNational InstituteDifferent endemic areasChronic relapsingFrequent international travelOptimal therapyClinical spectrumMultiple lesionsAmerican patientsLeishmaniasisPatientsLeishmania majorDiseaseLesionsEarly ageInternational travelU.S. residentsHealthRelapsingMajorityTherapyCutaneous leishmaniasis in western Venezuela caused by infection with Leishmania venezuelensis and L. braziliensis variants
Bonfante-Garrido R, Meléndez E, Barroeta S, de Alejos M, Momen H, Cupolillo E, McMahon-Pratt D, Grimaldi G. Cutaneous leishmaniasis in western Venezuela caused by infection with Leishmania venezuelensis and L. braziliensis variants. Transactions Of The Royal Society Of Tropical Medicine And Hygiene 1992, 86: 141-148. PMID: 1440772, DOI: 10.1016/0035-9203(92)90544-m.Peer-Reviewed Original ResearchConceptsL. braziliensisEndemic regionsSame endemic regionLeishmania venezuelensisL. venezuelensisSpecific monoclonal antibodiesCutaneous leishmaniasisPositive culturesEpidemiological studiesInfected donkeysHuman casesMonoclonal antibodiesDomestic reservoirBraziliensisL. guyanensisVenezuelensisState of LaraIsoenzyme electrophoresisReference strains
1985
Mucocutaneous leishmaniasis in Colombia: Leishmania braziliensis subspecies diversity.
Saravia N, Holguín A, D'Alessandro A, McMahon-Pratt D. Mucocutaneous leishmaniasis in Colombia: Leishmania braziliensis subspecies diversity. American Journal Of Tropical Medicine And Hygiene 1985, 34: 714-20. PMID: 4025684, DOI: 10.4269/ajtmh.1985.34.714.Peer-Reviewed Original ResearchConceptsMucocutaneous leishmaniasisLeishmania braziliensis braziliensisCutaneous manifestationsMucocutaneous diseaseMucocutaneous lesionsMucosal lesionsMetastatic developmentDisease formsEtiologic agentLeishmania braziliensisLeishmania isolatesLeishmaniasisLesionsBraziliensisSpecificity characteristicsParasitesPatientsDisease