2000
The Immunologically Protective P-4 Antigen ofLeishmania Amastigotes A DEVELOPMENTALLY REGULATED SINGLE STRAND-SPECIFIC NUCLEASE ASSOCIATED WITH THE ENDOPLASMIC RETICULUM*
Kar S, Soong L, Colmenares M, Goldsmith-Pestana K, McMahon-Pratt D. The Immunologically Protective P-4 Antigen ofLeishmania Amastigotes A DEVELOPMENTALLY REGULATED SINGLE STRAND-SPECIFIC NUCLEASE ASSOCIATED WITH THE ENDOPLASMIC RETICULUM*. Journal Of Biological Chemistry 2000, 275: 37789-37797. PMID: 10969068, DOI: 10.1074/jbc.m002149200.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumSpecific nuclease activityBlot analysisImmunofluorescence microscopic analysisIntracellular amastigote stageProtein sequence dataSingle-strand nucleasePeripheral blood mononuclear cellsSouthern blot analysisNorthern blot analysisBlood mononuclear cellsMembrane proteinsRNA stabilitySubcellular localizationFuture vaccine studiesDNA repairSequence dataTerminal domainAmerican cutaneous leishmaniasisSequence analysisNuclease activityAmastigote stagePromastigote stageSpecific nucleasesT helper
1996
Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms
Hodgkinson V, Soong L, Duboise S, McMahon-Pratt D. Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms. Experimental Parasitology 1996, 83: 94-105. PMID: 8654556, DOI: 10.1006/expr.1996.0053.Peer-Reviewed Original ResearchConceptsAmastigote-like formsNorthern blot analysisDevelopmental biologyContinual cultureAxenic cultureMolecular biologyTransformation of promastigotesOrganismsDNA probesGeneration timeCultured amastigotesAxenic cultivationBlot analysisExpression levelsSpecies of LeishmaniaSpeciesProtein reactiveBiologyDegrees CFirst reportPeriod of growthAmastigotesPromastigotesLeishmania amazonensisCultivation