2022
Longitudinal single-cell analysis of a patient receiving adoptive cell therapy reveals potential mechanisms of treatment failure
Qu R, Kluger Y, Yang J, Zhao J, Hafler D, Krause D, Bersenev A, Bosenberg M, Hurwitz M, Lucca L, Kluger H. Longitudinal single-cell analysis of a patient receiving adoptive cell therapy reveals potential mechanisms of treatment failure. Molecular Cancer 2022, 21: 219. PMID: 36514045, PMCID: PMC9749221, DOI: 10.1186/s12943-022-01688-5.Peer-Reviewed Original ResearchConceptsAdoptive cell therapySingle-cell analysisDepth single-cell analysisSingle-cell RNAACT productsDisease progressionT-cell receptor sequencingCell therapyFamily genesFeatures of exhaustionMultiple tumor typesCell expansionGenesNew clonotypesTIL preparationsClonal cell expansionCytokine therapyTreatment failureSerial bloodClonesEffector functionsSerial samplesTumor typesCellular therapyTherapy
2019
A versatile flow-based assay for immunocyte-mediated cytotoxicity
Rabinovich PM, Zhang J, Kerr SR, Cheng BH, Komarovskaya M, Bersenev A, Hurwitz ME, Krause DS, Weissman SM, Katz SG. A versatile flow-based assay for immunocyte-mediated cytotoxicity. Journal Of Immunological Methods 2019, 474: 112668. PMID: 31525367, PMCID: PMC6891822, DOI: 10.1016/j.jim.2019.112668.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TumorCell NucleusCytotoxicity Tests, ImmunologicCytotoxicity, ImmunologicFlow CytometryHigh-Throughput Screening AssaysHumansImmunotherapy, AdoptiveKiller Cells, NaturalLymphocytes, Tumor-InfiltratingMaleMelanomaMice, Inbred C57BLPredictive Value of TestsReceptors, Chimeric AntigenReproducibility of ResultsSkin NeoplasmsTime FactorsT-LymphocytesWorkflowConceptsCell-mediated cytotoxicityTumor-Infiltrating LymphocytesEffector cellsTarget cellsNK-92 cellsChimeric antigen receptorNuclear staining patternInfiltrating lymphocytesT cellsEffector nucleiFlow-based assayImmune systemFlow cytometryStaining patternAntigen receptorDead cellsKilling reactionCytotoxicityCell permeable dyeCellsAssaysCell mixturesNuclear proteinsNovel strategyCell proteins