2013
Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells
Lawton BR, Sosa JA, Roman S, Krause DS. Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells. Stem Cell Reviews And Reports 2013, 9: 578-585. PMID: 23719997, DOI: 10.1007/s12015-013-9447-2.Peer-Reviewed Original ResearchMeSH KeywordsCell Culture TechniquesCell DifferentiationCell LineCell LineageCell MovementCell SurvivalCollagenDrug CombinationsEmbryonic Stem CellsEndodermFluorescent Antibody TechniqueGene Expression Regulation, DevelopmentalGlutamine-Fructose-6-Phosphate Transaminase (Isomerizing)Hepatocyte Nuclear Factor 3-alphaHepatocyte Nuclear Factor 3-betaHumansLamininProteoglycansReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionSOXF Transcription FactorsTime FactorsConceptsHuman embryonic stem cellsEmbryonic stem cellsDefinitive endodermEndodermal differentiationGene expression patternsStem cellsPrecardiac mesodermExpression patternsLow-serum mediumCell deathMesenchymal transitionMigratory characteristicsKey eventsEndodermDifferentiationCell viabilityActivin ASerum mediumCellsGastrulationImproved protocolMesodermInductionGenesNutrients
2012
Complex oncogene dependence in microRNA-125a–induced myeloproliferative neoplasms
Guo S, Bai H, Megyola CM, Halene S, Krause DS, Scadden DT, Lu J. Complex oncogene dependence in microRNA-125a–induced myeloproliferative neoplasms. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 16636-16641. PMID: 23012470, PMCID: PMC3478612, DOI: 10.1073/pnas.1213196109.Peer-Reviewed Original ResearchAnimalsBone Marrow CellsBone Marrow NeoplasmsBone Marrow TransplantationCell LineColony-Forming Units AssayDoxycyclineFlow CytometryGene Expression Regulation, NeoplasticGranulocyte-Macrophage Colony-Stimulating FactorInterleukin-3Leukocytes, MononuclearMiceMice, Inbred C57BLMicroRNAsMyeloproliferative DisordersOncogenesReverse Transcriptase Polymerase Chain Reaction
2010
Serum response factor is an essential transcription factor in megakaryocytic maturation
Halene S, Gao Y, Hahn K, Massaro S, Italiano JE, Schulz V, Lin S, Kupfer GM, Krause DS. Serum response factor is an essential transcription factor in megakaryocytic maturation. Blood 2010, 116: 1942-1950. PMID: 20525922, PMCID: PMC3173990, DOI: 10.1182/blood-2010-01-261743.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBleeding TimeBlood PlateletsBone Marrow CellsCell DifferentiationCell LineageCells, CulturedCytoskeletonFemaleFlow CytometryGene Expression ProfilingLuminescent ProteinsMaleMegakaryocytesMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicMicroscopy, Electron, TransmissionPlatelet CountPlatelet Factor 4Reverse Transcriptase Polymerase Chain ReactionSerum Response FactorThrombocytopeniaTranscription FactorsConceptsSerum response factorCytoskeletal genesTranscription factorsMADS-box transcription factorsRole of SRFNormal megakaryocyte maturationAbnormal actin distributionResponse factorEssential transcription factorNormal Mendelian frequencyMegakaryocyte developmentMuscle differentiationPF4-Cre miceStress fibersMegakaryocyte maturationMegakaryocytic maturationActin distributionMegakaryocytic lineageMendelian frequencyMegakaryocyte progenitorsVivo assaysCFU-MKGenesPlatelet productionCritical role
2004
Bone Marrow-Derived Cells Contribute to Epithelial Engraftment during Wound Healing
Borue X, Lee S, Grove J, Herzog EL, Harris R, Diflo T, Glusac E, Hyman K, Theise ND, Krause DS. Bone Marrow-Derived Cells Contribute to Epithelial Engraftment during Wound Healing. American Journal Of Pathology 2004, 165: 1767-1772. PMID: 15509544, PMCID: PMC1618655, DOI: 10.1016/s0002-9440(10)63431-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Marrow CellsCell DifferentiationCell ProliferationEpithelial CellsEpitheliumFemaleGreen Fluorescent ProteinsIn Situ Hybridization, FluorescenceKeratinocytesKeratinsKi-67 AntigenMaleMiceReverse Transcriptase Polymerase Chain ReactionSkinTime FactorsWound HealingConceptsBone marrow-derived cellsEpithelial cellsMarrow-derived epithelial cellsEngraftment of BMDCsDonor-derived cellsMarrow-derived cellsWound healingDegree of engraftmentLevel of engraftmentAbsence of injuryEarly wound healingFemale miceBone marrowCytokeratin 5Cre-lox systemEngraftmentSkin damageWound edgeKeratinocytesInjuryWound siteTransit-amplifying cellsStem cellsEpidermal stem cellsRecent findingsLack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia
Harris RG, Herzog EL, Bruscia EM, Grove JE, Van Arnam JS, Krause DS. Lack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia. Science 2004, 305: 90-93. PMID: 15232107, DOI: 10.1126/science.1098925.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta-GalactosidaseBone Marrow CellsBone Marrow TransplantationCell DifferentiationCell FusionCobra Cardiotoxin ProteinsElapid VenomsEpithelial CellsFemaleGreen Fluorescent ProteinsHepatocytesKeratinocytesKeratinsLuminescent ProteinsMaleMiceMice, TransgenicMuscle CellsRadiation, IonizingRecombinasesRecombination, GeneticReverse Transcriptase Polymerase Chain ReactionStem CellsX ChromosomeY ChromosomeConceptsCell-cell fusionBone marrow-derived cellsCre/lox systemGreen fluorescent protein expressionFluorescent protein expressionEpithelial cellsDevelopmental plasticityLox systemCell fusionProtein expressionMarrow-derived cellsTransgenic miceCellsBone marrowFusionFusion requirementsPlasticityExpression