2010
SENP1-mediated GATA1 deSUMOylation is critical for definitive erythropoiesis
Yu L, Ji W, Zhang H, Renda MJ, He Y, Lin S, Cheng EC, Chen H, Krause DS, Min W. SENP1-mediated GATA1 deSUMOylation is critical for definitive erythropoiesis. Journal Of Experimental Medicine 2010, 207: 1183-1195. PMID: 20457756, PMCID: PMC2882842, DOI: 10.1084/jem.20092215.Peer-Reviewed Original ResearchConceptsSmall ubiquitin-like modifier (SUMO) modificationImportant regulatory mechanismEmbryonic day 13.5Down-regulation correlatesFetal liverCre-loxP systemEmbryonic lethalityProtein functionDefinitive erythropoiesisGene promoterDNA bindingRegulatory mechanismsGene expressionGATA1SENP1Fetal liver cellsProtein analysisDay 13.5Global deletionProteinSubsequent erythropoiesisKnockout miceErythropoiesisLiver cellsDeSUMOylation
2009
Dynamics of α-globin locus chromatin structure and gene expression during erythroid differentiation of human CD34+ cells in culture
Mahajan MC, Karmakar S, Newburger PE, Krause DS, Weissman SM. Dynamics of α-globin locus chromatin structure and gene expression during erythroid differentiation of human CD34+ cells in culture. Experimental Hematology 2009, 37: 1143-1156.e3. PMID: 19607874, PMCID: PMC2997688, DOI: 10.1016/j.exphem.2009.07.001.Peer-Reviewed Original ResearchMeSH KeywordsAlpha-GlobinsAntigens, CD34CCCTC-Binding FactorCells, CulturedChromatin Assembly and DisassemblyEnhancer Elements, GeneticErythroid Precursor CellsErythropoiesisErythropoietinGATA1 Transcription FactorGene Expression Regulation, DevelopmentalGlycophorinsHematopoietic Cell Growth FactorsHistonesHumansInsulator ElementsNF-E2 Transcription Factor, p45 SubunitProtein BindingRepressor ProteinsRNA Polymerase IITranscription FactorsConceptsAlpha-globin lociTranscription factor recruitmentChromatin structureGATA-1Transcription factorsErythroid differentiationGene expressionFactor recruitmentPol IIQuantitative polymerase chain reaction analysisAlpha-globin gene expressionKey erythroid transcription factorsErythroid transcription factorsNF-E2Chromatin immunoprecipitation-quantitative polymerase chain reaction analysisAlpha-globin genesUpstream activator sitesBeta-like genesPolymerase chain reaction analysisChain reaction analysisStages of erythropoiesisGlobin promoterDifferent differentiation stagesFactor CTCFHistone modifications