1999
A Nuclear Factor Y (NFY) Site Positively Regulates the Human CD34 Stem Cell Gene
Radomska H, Satterthwaite A, Taranenko N, Narravula S, Krause D, Tenen D. A Nuclear Factor Y (NFY) Site Positively Regulates the Human CD34 Stem Cell Gene. Blood 1999, 94: 3772-3780. DOI: 10.1182/blood.v94.11.3772.Peer-Reviewed Original ResearchHuman CD34 geneCD34 geneUntranslated regionC-MybPromoter activityCell-cycle regulated expressionMurine CD34 geneC-Myb binding sitesElectrophoretic mobility shift assaysNuclear factor Y (NF-Y) sitesTranscription factor NFYCCAAT box motifStem cell genesMobility shift assaysOptimal promoter activityReporter gene activityAntibody supershift experimentsEarly hematopoietic cellsTransient transfection experimentsBox motifCombinatorial actionGene activityPromoter functionOligonucleotide competitionShift assaysA nuclear factor Y (NFY) site positively regulates the human CD34 stem cell gene.
Radomska H, Satterthwaite A, Taranenko N, Narravula S, Krause D, Tenen D. A nuclear factor Y (NFY) site positively regulates the human CD34 stem cell gene. Blood 1999, 94: 3772-80. PMID: 10572091, DOI: 10.1182/blood.v94.11.3772.423k19_3772_3780.Peer-Reviewed Original ResearchConceptsHuman CD34 geneUntranslated regionCD34 geneC-MybPromoter activityCell-cycle regulated expressionMurine CD34 geneC-Myb binding sitesElectrophoretic mobility shift assaysNuclear factor Y (NF-Y) sitesTranscription factor NFYCCAAT box motifStem cell genesMobility shift assaysOptimal promoter activityReporter gene activityAntibody supershift experimentsEarly hematopoietic cellsTransient transfection experimentsBox motifCombinatorial actionGene activityPromoter functionOligonucleotide competitionCis elements
1998
CD34 expression by embryonic hematopoietic and endothelial cells does not require c-Myb.
Krause DS, Mucenski ML, Lawler AM, May WS. CD34 expression by embryonic hematopoietic and endothelial cells does not require c-Myb. Experimental Hematology 1998, 26: 1086-92. PMID: 9766450.Peer-Reviewed Original ResearchConceptsC-MybDevelopmental hematopoiesisES cellsPromoter activityHematopoietic cellsD3 embryonic stem cellsStage-specific mannerES cell differentiationEmbryonic stem cellsPrimitive hematopoietic cellsDefinitive hematopoiesisBone marrow stemCell surface glycoproteinBlood islandsEmbryoid bodiesEndothelial cellsTransient transfectionCell differentiationEmbryosStem cellsProgenitor cellsYolk sacHematopoiesisNonhematopoietic cellsCD34 expression