2010
Dantrolene mitigates caerulein-induced pancreatitis in vivo in mice
Orabi AI, Shah AU, Ahmad MU, Choo-Wing R, Parness J, Jain D, Bhandari V, Husain SZ. Dantrolene mitigates caerulein-induced pancreatitis in vivo in mice. AJP Gastrointestinal And Liver Physiology 2010, 299: g196-g204. PMID: 20448143, PMCID: PMC2904115, DOI: 10.1152/ajpgi.00498.2009.Peer-Reviewed Original ResearchMeSH KeywordsAmylasesAnimalsApoptosisCalcium Channel BlockersCalcium SignalingCeruletideCytoprotectionDantroleneDisease Models, AnimalEnzyme ActivationFluorescent Antibody TechniqueIn Situ Nick-End LabelingMaleMiceMice, Inbred C57BLMicroscopy, ElectronPancreasPancreatitisRyanodine Receptor Calcium Release ChannelSeverity of Illness IndexTime FactorsTrypsinConceptsPancreatic trypsin activityCaerulein-induced pancreatitisRyanodine receptorAcinar cellsMouse pancreatic sectionsHigh-risk settingsTUNEL-positive cellsLater time pointsRyR inhibitor dantroleneDantrolene pretreatmentHistological severityAcute pancreatitisPancreatic acinar cellsInflammatory disordersProphylactic treatmentRyR inhibitionSerum amylasePancreatitis inductionPancreatic sectionsPancreatitisAdequate treatmentTrypsin activityEarly markerCaerulein hyperstimulationUltrastructural derangements
2009
Protease activation during in vivo pancreatitis is dependent on calcineurin activation
Shah AU, Sarwar A, Orabi AI, Gautam S, Grant WM, Park AJ, Shah AU, Liu J, Mistry PK, Jain D, Husain SZ. Protease activation during in vivo pancreatitis is dependent on calcineurin activation. AJP Gastrointestinal And Liver Physiology 2009, 297: g967-g973. PMID: 20501444, PMCID: PMC2777459, DOI: 10.1152/ajpgi.00181.2009.Peer-Reviewed Original ResearchConceptsAcinar cellsProtease activationDependent phosphatase calcineurinHourly intraperitoneal injectionsCourse of pancreatitisAcinar cell vacuolizationCholecystokinin analogue caeruleinPhosphatase calcineurinHistological severityMyeloperoxidase activityPancreatic acinar cellsAcute pancreatitisIL-6Inhibitor FK506Pancreatic edemaSerum amylaseIntraperitoneal injectionPancreatitis inductionPancreatitis severityCalcineurin activationPancreatitisPathological riseCell vacuolizationDigestive proenzymesPremature activation