2017
STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells
Erdmann RS, Toomre D, Schepartz A. STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells. Methods In Molecular Biology 2017, 1663: 65-78. PMID: 28924659, PMCID: PMC6146391, DOI: 10.1007/978-1-4939-7265-4_6.Peer-Reviewed Original ResearchMeSH KeywordsCeramidesDrug StabilityFluorescent DyesGolgi ApparatusHeLa CellsHumansImaging, Three-DimensionalMicroscopy, FluorescenceRhodaminesConceptsLive cellsMembrane-bound proteinsLipid probesGolgi dynamicsCellular functionsGolgi structureCellular organellesGolgi apparatusCeramide lipidsSuper-resolution imagingLabeling strategySTED imagingSTED microscopyCellsPhotostable fluorophoresLipidsGolgiOrganellesTwo-componentBioorthogonal reactionsProbeProteinHigh density
2011
3-D Reconstruction of Microtubules from Multi-Angle Total Internal Reflection Fluorescence Microscopy Using Bayesian Framework
Yang Q, Karpikov A, Toomre D, Duncan JS. 3-D Reconstruction of Microtubules from Multi-Angle Total Internal Reflection Fluorescence Microscopy Using Bayesian Framework. IEEE Transactions On Image Processing 2011, 20: 2248-2259. PMID: 21324778, DOI: 10.1109/tip.2011.2114359.Peer-Reviewed Original ResearchConceptsEvanescent fieldDifferent penetration depthsTIRF imagesPenetration depthSamples of microtubulesLaser beamTotal internal reflection fluorescence microscopyAxial resolutionReflection fluorescence microscopyLarge radiusIncident angleMulti-angle total internal reflection fluorescence microscopyElectron microscopy imagesTIRF dataSmall radiusTracking of microtubulesMicroscopy imagesFluorescence microscopyMicroscopyRadiusReconstruction resultsCurvilinear characteristicsZ-dimensionExperimental calibrationMicrotubule curvature
2010
Estimation of 3D Geometry of Microtubules Using Multi-angle Total Internal Reflection Fluorescence Microscopy
Yang Q, Karpikov A, Toomre D, Duncan J. Estimation of 3D Geometry of Microtubules Using Multi-angle Total Internal Reflection Fluorescence Microscopy. Lecture Notes In Computer Science 2010, 13: 538-545. PMID: 20879357, DOI: 10.1007/978-3-642-15745-5_66.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyReflection fluorescence microscopyTotal internal reflection fluorescence microscopy imagesPtK2 cellsMicrotubulesFluorescence microscopyTIRF imagesImportant biological parametersTIRF dataFluorescence microscopy imagesMulti-angle total internal reflection fluorescence microscopyBiological parametersMicrotubule curvatureBiological samples
2005
Segmentation and 3D Reconstruction of Microtubules in Total Internal Reflection Fluorescence Microscopy (TIRFM)
Hadjidemetriou S, Toomre D, Duncan JS. Segmentation and 3D Reconstruction of Microtubules in Total Internal Reflection Fluorescence Microscopy (TIRFM). Lecture Notes In Computer Science 2005, 8: 761-769. PMID: 16685915, DOI: 10.1007/11566465_94.Peer-Reviewed Original Research