2010
Estimation of 3D Geometry of Microtubules Using Multi-angle Total Internal Reflection Fluorescence Microscopy
Yang Q, Karpikov A, Toomre D, Duncan J. Estimation of 3D Geometry of Microtubules Using Multi-angle Total Internal Reflection Fluorescence Microscopy. Lecture Notes In Computer Science 2010, 13: 538-545. PMID: 20879357, DOI: 10.1007/978-3-642-15745-5_66.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyReflection fluorescence microscopyTotal internal reflection fluorescence microscopy imagesPtK2 cellsMicrotubulesFluorescence microscopyTIRF imagesImportant biological parametersTIRF dataFluorescence microscopy imagesMulti-angle total internal reflection fluorescence microscopyBiological parametersMicrotubule curvatureBiological samples
2008
Motion tracking of the outer tips of microtubules
Hadjidemetriou S, Toomre D, Duncan J. Motion tracking of the outer tips of microtubules. Medical Image Analysis 2008, 12: 689-702. PMID: 18571462, DOI: 10.1016/j.media.2008.04.004.Peer-Reviewed Original ResearchConceptsMicrotubule assemblyCoordination of mitosisTransport of chromosomesHigh throughput quantitative studiesNumerous critical rolesMicrotubule tipsLiving cellsCell migrationStructural tracksConfocal microscopyEpifluorescent microscopyNeurodegenerative diseasesCritical roleCell pathologyMicrotubulesAssemblyCellsSequenceOuter tipChromosomesOrganellesMitosisAbnormal functionCytoplasmVesicles
2003
Nanometer targeting of microtubules to focal adhesions
Krylyshkina O, Anderson KI, Kaverina I, Upmann I, Manstein DJ, Small JV, Toomre DK. Nanometer targeting of microtubules to focal adhesions. Journal Of Cell Biology 2003, 161: 853-859. PMID: 12782685, PMCID: PMC2172972, DOI: 10.1083/jcb.200301102.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyAdhesion complexesIntact microtubule cytoskeletonReflection fluorescence microscopyGFP-CLIP-170Substrate adhesionFocal adhesionsMicrotubule cytoskeletonCell movementCell peripheryCommon cytoskeletal elementsMicrotubule tipsAdhesion fociCytoskeletal elementsPolymerizing microtubulesTip complexGFP-tubulinMicrotubule endsFluorescence microscopyMicrotubulesMultiple microtubulesSite targetingCentral roleComplexesTargeting