2016
The IN/OUT assay: a new tool to study ciliogenesis
Kukic I, Rivera-Molina F, Toomre D. The IN/OUT assay: a new tool to study ciliogenesis. Cilia 2016, 5: 23. PMID: 27493724, PMCID: PMC4972980, DOI: 10.1186/s13630-016-0044-2.Peer-Reviewed Original ResearchCiliary pocketCiliary transmembrane proteinsPlasma membrane invaginationsDeep plasma membrane invaginationsPrimary cilia assemblyCilia assemblyMembrane invaginationsTransmembrane proteinPrimary ciliaCellular antennaCilia lengthMolecular characterizationMost ciliaFunctional studiesCiliogenesisCiliaNew toolRPE cellsCellsAssaysAntibody accessEHD1New assayPocketConventional assays
2006
Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages
Perera RM, Zoncu R, Lucast L, De Camilli P, Toomre D. Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 19332-19337. PMID: 17158794, PMCID: PMC1693868, DOI: 10.1073/pnas.0609795104.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopySynaptojanin 1CCP formationMulticolor total internal reflection fluorescence microscopyClathrin-coated pit dynamicsClathrin-coated pitsClathrin-dependent endocytosisEndocytic clathrin adaptorsSynaptic vesicle recyclingReflection fluorescence microscopyCell-free systemClathrin adaptorsPolyphosphoinositide phosphataseGenetic manipulationVesicle recyclingPit dynamicsIntact cellsSplice variantsFunctional studiesFluorescence microscopyEndophilinTemporal recruitmentDirect interactionIsoformsPredominant isoform