2009
A Phosphoinositide Switch Controls the Maturation and Signaling Properties of APPL Endosomes
Zoncu R, Perera RM, Balkin DM, Pirruccello M, Toomre D, De Camilli P. A Phosphoinositide Switch Controls the Maturation and Signaling Properties of APPL Endosomes. Cell 2009, 136: 1110-1121. PMID: 19303853, PMCID: PMC2705806, DOI: 10.1016/j.cell.2009.01.032.Peer-Reviewed Original ResearchConceptsPositive endosomesAPPL endosomesEndocytic pathwayGrowth factor receptor traffickingEarly endocytic pathwayRab5-positive endosomesSingle-vesicle imagingGrowth factor signalingNovel endocytic compartmentsSurprising plasticityEndocytic vesiclesFactor signalingEndocytic compartmentsEnhanced growth factor signalingReceptor traffickingEndosomesSignaling propertiesRecent identificationPI3PCritical roleCurrent understandingSignalingPathwayFunctional organizationMaturation
2008
Motion tracking of the outer tips of microtubules
Hadjidemetriou S, Toomre D, Duncan J. Motion tracking of the outer tips of microtubules. Medical Image Analysis 2008, 12: 689-702. PMID: 18571462, DOI: 10.1016/j.media.2008.04.004.Peer-Reviewed Original ResearchConceptsMicrotubule assemblyCoordination of mitosisTransport of chromosomesHigh throughput quantitative studiesNumerous critical rolesMicrotubule tipsLiving cellsCell migrationStructural tracksConfocal microscopyEpifluorescent microscopyNeurodegenerative diseasesCritical roleCell pathologyMicrotubulesAssemblyCellsSequenceOuter tipChromosomesOrganellesMitosisAbnormal functionCytoplasmVesicles