2013
Live-cell imaging of exocyst links its spatiotemporal dynamics to various stages of vesicle fusion
Rivera-Molina F, Toomre D. Live-cell imaging of exocyst links its spatiotemporal dynamics to various stages of vesicle fusion. Journal Of Cell Biology 2013, 201: 673-680. PMID: 23690179, PMCID: PMC3664709, DOI: 10.1083/jcb.201212103.Peer-Reviewed Original ResearchConceptsLive-cell imagingVesicle fusionEndocytic recycling compartmentAttachment protein receptorsSNARE fusion machineryExocyst complexMembrane traffickingFusion machineryRecycling compartmentCell cortexSec8Cell protrusionsPlasma membraneVesicle attachmentFusion poreMembrane expansionProtein receptorsCell polarizationFluorescence recoverySpatiotemporal dynamicsExocystUbiquitous roleVesiclesTraffickingMorphological criteria
2010
Deciphering subcellular processes in live imaging datasets via dynamic probabilistic networks
Letinic K, Sebastian R, Barthel A, Toomre D. Deciphering subcellular processes in live imaging datasets via dynamic probabilistic networks. Bioinformatics 2010, 26: 2029-2036. PMID: 20581401, PMCID: PMC2916721, DOI: 10.1093/bioinformatics/btq331.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyCellular statesDistinct cellular statesSpatial-temporal regulationNon-polarized cellsComplex intracellular processesReflection fluorescence microscopyLive cell imaging dataOrganelle behaviorGLUT4 vesiclesProtein complexesCellular processesSpatial regulationDevelopmental processesBiological processesSubcellular processesCell imaging dataCell polarizationLiving cellsIntracellular processesBlood glucose homeostasisFluorescence microscopyExocytosisCell imagingStatic snapshots