2017
Characteristics of allelic gene expression in human brain cells from single-cell RNA-seq data analysis
Zhao D, Lin M, Pedrosa E, Lachman HM, Zheng D. Characteristics of allelic gene expression in human brain cells from single-cell RNA-seq data analysis. BMC Genomics 2017, 18: 860. PMID: 29126398, PMCID: PMC5681780, DOI: 10.1186/s12864-017-4261-x.Peer-Reviewed Original ResearchMeSH KeywordsAllelesBrainGene Expression ProfilingHumansPolymorphism, Single NucleotideSequence Analysis, RNASingle-Cell AnalysisConceptsMonoallelic expressionHuman brain cellsGene expressionMonoallelic gene expressionAllelic gene expressionGenome-wide levelSingle-cell RNA-seq datasetsRNA-seq data analysisAllelic expression studiesSingle-cell RNA-seq data analysisRNA-seq datasetsSingle nucleotide variantsBrain cellsCellular identityAutosomal genesNeuronal diversityExpression studiesNucleotide variantsCorrelated expressionGenesIndividual cellsHuman psychiatric disordersNeuronal cellsSingle cellsCell functionTranscriptome analysis of microglia in a mouse model of Rett syndrome: differential expression of genes associated with microglia/macrophage activation and cellular stress
Zhao D, Mokhtari R, Pedrosa E, Birnbaum R, Zheng D, Lachman HM. Transcriptome analysis of microglia in a mouse model of Rett syndrome: differential expression of genes associated with microglia/macrophage activation and cellular stress. Molecular Autism 2017, 8: 17. PMID: 28367307, PMCID: PMC5372344, DOI: 10.1186/s13229-017-0134-z.Peer-Reviewed Original ResearchConceptsHeat shock protein familyShock protein familyWhole transcriptome analysisChromatin regulatorsAnalysis of microgliaPhenotypic phasesTranscriptome analysisProtein familyRNA-seqCellular stressMethyl-CpGRTT phenotypeRTT pathogenesisDe novo lossGene expressionM2 activation statesFemale miceDifferential expressionFunction mutationsHSP pathwayGenesMolecular pathwaysCell typesExtracellular matrixProtein 2
2015
MicroRNA Profiling of Neurons Generated Using Induced Pluripotent Stem Cells Derived from Patients with Schizophrenia and Schizoaffective Disorder, and 22q11.2 Del
Zhao D, Lin M, Chen J, Pedrosa E, Hrabovsky A, Fourcade HM, Zheng D, Lachman HM. MicroRNA Profiling of Neurons Generated Using Induced Pluripotent Stem Cells Derived from Patients with Schizophrenia and Schizoaffective Disorder, and 22q11.2 Del. PLOS ONE 2015, 10: e0132387. PMID: 26173148, PMCID: PMC4501820, DOI: 10.1371/journal.pone.0132387.Peer-Reviewed Original ResearchConceptsGenome-wide significanceUnderlying genetic basisInduced pluripotent stem cellsPluripotent stem cell (iPSC) technologyMiRNA expression profilingPluripotent stem cellsMiRNA expression patternsMicroRNA biogenesisMRNA targetsRegulated miRNAsGenetic basisExpression profilingStem cell technologyExpression patternsAutopsy samplesMiRNAsNeuropsychiatric disordersMicroRNA profilingStem cellsNominal significanceGenesPeripheral cellsPeripheral bloodWider significanceGenetic factors