2020
Minimally Invasive Fetal Therapy for Myelomeningocele
Freedman-Weiss M, Stitelman D. Minimally Invasive Fetal Therapy for Myelomeningocele. Current Stem Cell Reports 2020, 6: 1-5. DOI: 10.1007/s40778-020-00167-1.Peer-Reviewed Original ResearchNon-invasive therapeutic approachInvasive fetal therapyPostnatal surgical repairDevastating clinical manifestationsTissue engineeringScaffold-based tissue engineeringMedical managementSurgical repairCell-based therapiesClinical manifestationsFetal stem cellsCurrent therapiesPatient outcomesSpinal cordFetal therapyFetal surgeryTherapeutic approachesTherapyInvasive wayTranslational scienceStem cellsReview detailsParticle deliveryNovel scientific researchParticle injection
2015
Life-Long Transgene Expression in Skeletal Muscle Without Transduction of Satellite Cells Following Embryonic Myogenic Progenitor Transduction by Lentivirus Administered in Utero
Stitelman DH, Brazelton TR, Endo M, Bora A, Traas J, Zoltick PW, Flake AW. Life-Long Transgene Expression in Skeletal Muscle Without Transduction of Satellite Cells Following Embryonic Myogenic Progenitor Transduction by Lentivirus Administered in Utero. Stem Cells And Development 2015, 24: 1878-1887. PMID: 25915576, PMCID: PMC4533031, DOI: 10.1089/scd.2014.0585.Peer-Reviewed Original ResearchConceptsSatellite cellsMarker gene expressionMyogenic stem cellsMammalian myogenesisTransgene expressionMuscle progenitorsPrimitive streakMyogenic progenitorsCommon progenitorGene expressionMarker transgeneAdult muscleGene transferTransductionStem cellsCell transductionDermomyotomeProgenitorsSkeletal muscleMuscle fibersSustained transgene expressionExpressionDermatomyotomeCellsRecent evidence
2014
Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9
Stitelman DH, Brazelton T, Bora A, Traas J, Merianos D, Limberis M, Davey M, Flake AW. Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9. Molecular Therapy — Methods & Clinical Development 2014, 1: 14040. PMID: 26015979, PMCID: PMC4362369, DOI: 10.1038/mtm.2014.40.Peer-Reviewed Original ResearchSatellite cell migrationGene transferCell migrationSatellite cellsMuscle stem cellsGreen fluorescent protein marker geneProtein marker geneMuscle satellite cellsEfficient gene expressionSkeletal muscle expressionMarker genesGene expressionDevelopmental stagesDifferentiated myofibersMyofiber isolationCellular migrationEfficient gene transferSatellite cell expressionAAV-9Stem cellsConfocal microscopyCell transductionMuscle expressionUtero deliveryTransduction
2010
Robust In Vivo Transduction of Nervous System and Neural Stem Cells by Early Gestational Intra Amniotic Gene Transfer Using Lentiviral Vector
Stitelman DH, Endo M, Bora A, Muvarak N, Zoltick PW, Flake AW, Brazelton TR. Robust In Vivo Transduction of Nervous System and Neural Stem Cells by Early Gestational Intra Amniotic Gene Transfer Using Lentiviral Vector. Molecular Therapy 2010, 18: 1615-1623. PMID: 20571539, PMCID: PMC2956924, DOI: 10.1038/mt.2010.125.Peer-Reviewed Original ResearchConceptsNeural stem cellsAdult neural stem cellsCell typesGene transferStem cellsGreen fluorescent protein marker geneProtein marker geneNervous system developmentNeural cell typesMajor neural cell typesLentiviral vectorsNeural cell populationsMajor cell typesAdult nervous systemGene functionMarker genesEmbryonic day 8Subventricular zoneEntire nervous systemNervous systemVivo transductionCell populationsFuture clinical applicationsGenetic disordersNeural groove
2004
Mechanism of angiopoietin-1 mediated endothelial progenitor cell recruitment in wound healing
Keswani S, Parvadia J, Stitelman D, Kozin E, Radu A, Alaee D, Zoltick P, Crombleholme T. Mechanism of angiopoietin-1 mediated endothelial progenitor cell recruitment in wound healing. Journal Of The American College Of Surgeons 2004, 199: 60. DOI: 10.1016/j.jamcollsurg.2004.05.124.Peer-Reviewed Original ResearchEPC recruitmentMMP9-/- miceAng-1Control miceWound healingEndothelial progenitor cell recruitmentSerum SCF levelsPotent therapeutic strategyProgenitor cell recruitmentCell recruitmentMMP9 activationSCF supplementationTherapeutic strategiesBone marrowAngiopoietin-1Enhanced neovascularizationEpithelial gapsNeovascularizationEndothelial progenitorsEPC/WoundsHealingSignificant differencesMiceStem cells