2015
Life-Long Transgene Expression in Skeletal Muscle Without Transduction of Satellite Cells Following Embryonic Myogenic Progenitor Transduction by Lentivirus Administered in Utero
Stitelman DH, Brazelton TR, Endo M, Bora A, Traas J, Zoltick PW, Flake AW. Life-Long Transgene Expression in Skeletal Muscle Without Transduction of Satellite Cells Following Embryonic Myogenic Progenitor Transduction by Lentivirus Administered in Utero. Stem Cells And Development 2015, 24: 1878-1887. PMID: 25915576, PMCID: PMC4533031, DOI: 10.1089/scd.2014.0585.Peer-Reviewed Original ResearchConceptsSatellite cellsMarker gene expressionMyogenic stem cellsMammalian myogenesisTransgene expressionMuscle progenitorsPrimitive streakMyogenic progenitorsCommon progenitorGene expressionMarker transgeneAdult muscleGene transferTransductionStem cellsCell transductionDermomyotomeProgenitorsSkeletal muscleMuscle fibersSustained transgene expressionExpressionDermatomyotomeCellsRecent evidence
2014
Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9
Stitelman DH, Brazelton T, Bora A, Traas J, Merianos D, Limberis M, Davey M, Flake AW. Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9. Molecular Therapy — Methods & Clinical Development 2014, 1: 14040. PMID: 26015979, PMCID: PMC4362369, DOI: 10.1038/mtm.2014.40.Peer-Reviewed Original ResearchSatellite cell migrationGene transferCell migrationSatellite cellsMuscle stem cellsGreen fluorescent protein marker geneProtein marker geneMuscle satellite cellsEfficient gene expressionSkeletal muscle expressionMarker genesGene expressionDevelopmental stagesDifferentiated myofibersMyofiber isolationCellular migrationEfficient gene transferSatellite cell expressionAAV-9Stem cellsConfocal microscopyCell transductionMuscle expressionUtero deliveryTransduction