2015
Life-Long Transgene Expression in Skeletal Muscle Without Transduction of Satellite Cells Following Embryonic Myogenic Progenitor Transduction by Lentivirus Administered in Utero
Stitelman DH, Brazelton TR, Endo M, Bora A, Traas J, Zoltick PW, Flake AW. Life-Long Transgene Expression in Skeletal Muscle Without Transduction of Satellite Cells Following Embryonic Myogenic Progenitor Transduction by Lentivirus Administered in Utero. Stem Cells And Development 2015, 24: 1878-1887. PMID: 25915576, PMCID: PMC4533031, DOI: 10.1089/scd.2014.0585.Peer-Reviewed Original ResearchConceptsSatellite cellsMarker gene expressionMyogenic stem cellsMammalian myogenesisTransgene expressionMuscle progenitorsPrimitive streakMyogenic progenitorsCommon progenitorGene expressionMarker transgeneAdult muscleGene transferTransductionStem cellsCell transductionDermomyotomeProgenitorsSkeletal muscleMuscle fibersSustained transgene expressionExpressionDermatomyotomeCellsRecent evidence
2014
Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9
Stitelman DH, Brazelton T, Bora A, Traas J, Merianos D, Limberis M, Davey M, Flake AW. Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9. Molecular Therapy — Methods & Clinical Development 2014, 1: 14040. PMID: 26015979, PMCID: PMC4362369, DOI: 10.1038/mtm.2014.40.Peer-Reviewed Original ResearchSatellite cell migrationGene transferCell migrationSatellite cellsMuscle stem cellsGreen fluorescent protein marker geneProtein marker geneMuscle satellite cellsEfficient gene expressionSkeletal muscle expressionMarker genesGene expressionDevelopmental stagesDifferentiated myofibersMyofiber isolationCellular migrationEfficient gene transferSatellite cell expressionAAV-9Stem cellsConfocal microscopyCell transductionMuscle expressionUtero deliveryTransduction
2010
Robust In Vivo Transduction of Nervous System and Neural Stem Cells by Early Gestational Intra Amniotic Gene Transfer Using Lentiviral Vector
Stitelman DH, Endo M, Bora A, Muvarak N, Zoltick PW, Flake AW, Brazelton TR. Robust In Vivo Transduction of Nervous System and Neural Stem Cells by Early Gestational Intra Amniotic Gene Transfer Using Lentiviral Vector. Molecular Therapy 2010, 18: 1615-1623. PMID: 20571539, PMCID: PMC2956924, DOI: 10.1038/mt.2010.125.Peer-Reviewed Original ResearchConceptsNeural stem cellsAdult neural stem cellsCell typesGene transferStem cellsGreen fluorescent protein marker geneProtein marker geneNervous system developmentNeural cell typesMajor neural cell typesLentiviral vectorsNeural cell populationsMajor cell typesAdult nervous systemGene functionMarker genesEmbryonic day 8Subventricular zoneEntire nervous systemNervous systemVivo transductionCell populationsFuture clinical applicationsGenetic disordersNeural groove
2009
The developmental stage determines the distribution and duration of gene expression after early intra-amniotic gene transfer using lentiviral vectors
Endo M, Henriques-Coelho T, Zoltick PW, Stitelman DH, Peranteau WH, Radu A, Flake AW. The developmental stage determines the distribution and duration of gene expression after early intra-amniotic gene transfer using lentiviral vectors. Gene Therapy 2009, 17: 61-71. PMID: 19727133, DOI: 10.1038/gt.2009.115.Peer-Reviewed Original ResearchConceptsGene transferAccessible stem cell populationGene expressionDevelopmental stagesEarly gestational time pointsGene expression correspondsStem cell populationGreen fluorescent protein expressionTissue expressionCell populationsFluorescent protein expressionEarly somite stagesEpithelial cellsGerm layersLentiviral vectorsOnly epithelial cellsDevelopmental diseasesNeural ectodermGFP expressionSomite stageEfficient gene transferExpression correspondsProtein expressionEctodermGestational time points