2014
Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9
Stitelman DH, Brazelton T, Bora A, Traas J, Merianos D, Limberis M, Davey M, Flake AW. Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9. Molecular Therapy — Methods & Clinical Development 2014, 1: 14040. PMID: 26015979, PMCID: PMC4362369, DOI: 10.1038/mtm.2014.40.Peer-Reviewed Original ResearchSatellite cell migrationGene transferCell migrationSatellite cellsMuscle stem cellsGreen fluorescent protein marker geneProtein marker geneMuscle satellite cellsEfficient gene expressionSkeletal muscle expressionMarker genesGene expressionDevelopmental stagesDifferentiated myofibersMyofiber isolationCellular migrationEfficient gene transferSatellite cell expressionAAV-9Stem cellsConfocal microscopyCell transductionMuscle expressionUtero deliveryTransduction
2009
The developmental stage determines the distribution and duration of gene expression after early intra-amniotic gene transfer using lentiviral vectors
Endo M, Henriques-Coelho T, Zoltick PW, Stitelman DH, Peranteau WH, Radu A, Flake AW. The developmental stage determines the distribution and duration of gene expression after early intra-amniotic gene transfer using lentiviral vectors. Gene Therapy 2009, 17: 61-71. PMID: 19727133, DOI: 10.1038/gt.2009.115.Peer-Reviewed Original ResearchConceptsGene transferAccessible stem cell populationGene expressionDevelopmental stagesEarly gestational time pointsGene expression correspondsStem cell populationGreen fluorescent protein expressionTissue expressionCell populationsFluorescent protein expressionEarly somite stagesEpithelial cellsGerm layersLentiviral vectorsOnly epithelial cellsDevelopmental diseasesNeural ectodermGFP expressionSomite stageEfficient gene transferExpression correspondsProtein expressionEctodermGestational time points