2024
Correction: Methylglyoxal, a glycolysis side-product, induces Hsp90 glycation and YAP-mediated tumor growth and metastasis
Nokin M, Durieux F, Peixoto P, Chiavarina B, Peulen O, Blomme A, Turtoi A, Costanza B, Smargiasso N, Baiwir D, Scheijen J, Schalkwijk C, Leenders J, De Tullio P, Bianchi E, Thiry M, Uchida K, Spiegel D, Cochrane J, Hutton C, De Pauw E, Delvenne P, Belpomme D, Castronovo V, Bellahcène A. Correction: Methylglyoxal, a glycolysis side-product, induces Hsp90 glycation and YAP-mediated tumor growth and metastasis. ELife 2024, 13: e103327. PMID: 39312391, PMCID: PMC11419668, DOI: 10.7554/elife.103327.Peer-Reviewed Original ResearchBifunctional Molecules That Induce Both Targeted Degradation and Transcytosis of Extracellular Proteins in Brain Cells
Howell R, Wang S, Khambete M, McDonald D, Spiegel D. Bifunctional Molecules That Induce Both Targeted Degradation and Transcytosis of Extracellular Proteins in Brain Cells. Journal Of The American Chemical Society 2024, 146: 16404-16411. PMID: 38855935, DOI: 10.1021/jacs.3c13320.Peer-Reviewed Original ResearchLow-density lipoprotein receptor-related protein 1Targeted protein degradationTarget proteinsClathrin-mediated mechanismLipoprotein receptor-related protein 1Chloroalkane ligandTranscytosis of proteinsExtracellular proteinsPeptide motifsLysosomal localizationTargeted degradationHaloTag proteinCovalent taggingProtein degradationLysosomal proteolysisBlood-brain barrierProteinDegradable proteinCentral nervous systemProtein 1Bifunctional small moleculesEffective therapeutic strategyPrimary receptorTranscytosisStreptavidinCorrection: Methylglyoxal, a glycolysis side-product, induces Hsp90 glycation and YAP-mediated tumor growth and metastasis
Nokin M, Durieux F, Peixoto P, Chiavarina B, Peulen O, Blomme A, Turtoi A, Costanza B, Smargiasso N, Baiwir D, Scheijen J, Schalkwijk C, Leenders J, De Tullio P, Bianchi E, Thiry M, Uchida K, Spiegel D, Cochrane J, Hutton C, De Pauw E, Delvenne P, Belpomme D, Castronovo V, Bellahcène A. Correction: Methylglyoxal, a glycolysis side-product, induces Hsp90 glycation and YAP-mediated tumor growth and metastasis. ELife 2024, 13: e96613. PMID: 38319293, PMCID: PMC10846855, DOI: 10.7554/elife.96613.Peer-Reviewed Original Research
2016
Histones are Targets for Modification by Glucose‐Derived Methylglyoxal
Galligan J, Mitchener M, Wauchope O, Wang T, Rose K, Spiegel D, Marnett L. Histones are Targets for Modification by Glucose‐Derived Methylglyoxal. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.586.2.Peer-Reviewed Original ResearchDiabetic nephropathyMetabolic memoryHyperglycemia associated with diabetesCause of morbidityMg–HHigh-resolution mass spectrometryDN pathogenesisModel of hyperglycemiaHistone posttranslational modificationsCell culture modelLC-MS/MSDN patientsDiabetic patientsLC-MS/MS methodologyGlycemic controlHEK293 cellsCultured HEK293 cellsPosttranslational modificationsMgO modificationOxidative stressGlyoxalase 1PatientsInflammationMass spectrometryAdducts
2010
Grand Challenge Commentary: Synthetic immunology to engineer human immunity
Spiegel DA. Grand Challenge Commentary: Synthetic immunology to engineer human immunity. Nature Chemical Biology 2010, 6: 871-872. PMID: 21079593, DOI: 10.1038/nchembio.477.Peer-Reviewed Original ResearchA Biosynthetic Strategy for Re-engineering the Staphylococcus aureus Cell Wall with Non-native Small Molecules
Nelson JW, Chamessian AG, McEnaney PJ, Murelli RP, Kazmiercak B, Spiegel DA. A Biosynthetic Strategy for Re-engineering the Staphylococcus aureus Cell Wall with Non-native Small Molecules. ACS Chemical Biology 2010, 5: 1147-1155. PMID: 20923200, PMCID: PMC3003768, DOI: 10.1021/cb100195d.Peer-Reviewed Original ResearchConceptsCell wallCell wall engineeringCell-wall peptidoglycanWild-type bacteriaGram-positive bacterial pathogensPathogenic Gram-positive bacteriaStaphylococcus aureus cell wallBacterial cell wallBacterial cell surfaceEnzyme sortase ASmall moleculesGram-positive bacteriaWall peptidoglycanAureus cell wallMolecular handleBiosynthetic strategiesCell surfaceBacterial pathogensEndogenous levelsSortase AAzide-alkyne cycloaddition reactionChemical handlesWall engineeringBacteriaWidespread utilityA Remote Arene-Binding Site on Prostate Specific Membrane Antigen Revealed by Antibody-Recruiting Small Molecules
Zhang AX, Murelli RP, Barinka C, Michel J, Cocleaza A, Jorgensen WL, Lubkowski J, Spiegel DA. A Remote Arene-Binding Site on Prostate Specific Membrane Antigen Revealed by Antibody-Recruiting Small Molecules. Journal Of The American Chemical Society 2010, 132: 12711-12716. PMID: 20726553, PMCID: PMC2965167, DOI: 10.1021/ja104591m.Peer-Reviewed Original Research
2009
Chemical Control over Immune Recognition: A Class of Antibody-Recruiting Small Molecules That Target Prostate Cancer
Murelli RP, Zhang AX, Michel J, Jorgensen WL, Spiegel DA. Chemical Control over Immune Recognition: A Class of Antibody-Recruiting Small Molecules That Target Prostate Cancer. Journal Of The American Chemical Society 2009, 131: 17090-17092. PMID: 19888723, PMCID: PMC2794306, DOI: 10.1021/ja906844e.Peer-Reviewed Original ResearchConceptsSmall moleculesFundamental chemical principlesBifunctional small moleculesProstate-specific membrane antigenProstate cancer cellsProstate cancerChemical principlesBiological evaluationCrystallographic dataMoleculesAmerican male populationTernary complexLNCaP human prostate cancer cellsCancer cellsHuman effector cellsHuman prostate cancer cellsCancer-related deathAntibody-dependent killingClasses of antibodiesHigh therapeutic potentialNovel classAntibody-recruiting moleculesHuman immune systemAnti-DNP antibodiesEffector cellsAn Antibody-Recruiting Small Molecule That Targets HIV gp120
Parker CG, Domaoal RA, Anderson KS, Spiegel DA. An Antibody-Recruiting Small Molecule That Targets HIV gp120. Journal Of The American Chemical Society 2009, 131: 16392-16394. PMID: 19839582, PMCID: PMC2783809, DOI: 10.1021/ja9057647.Peer-Reviewed Original ResearchConceptsVirus entryComplement-mediated destructionNew treatment strategiesAntibody-recruiting small moleculeAnti-HIV activityHIV/AIDSViral glycoprotein gp120Env-expressing cellsHuman immune systemHuman T cellsSurface viral glycoproteinsHIV treatmentTreatment strategiesHIV survivalT cellsNovel small moleculesHIV gp120Glycoprotein gp120Immune systemGp120Virus replicationAntibodiesGlobal pandemicViral glycoproteinsVirus particles