A Biosynthetic Strategy for Re-engineering the Staphylococcus aureus Cell Wall with Non-native Small Molecules
Nelson JW, Chamessian AG, McEnaney PJ, Murelli RP, Kazmiercak B, Spiegel DA. A Biosynthetic Strategy for Re-engineering the Staphylococcus aureus Cell Wall with Non-native Small Molecules. ACS Chemical Biology 2010, 5: 1147-1155. PMID: 20923200, PMCID: PMC3003768, DOI: 10.1021/cb100195d.Peer-Reviewed Original ResearchConceptsCell wallCell wall engineeringCell-wall peptidoglycanWild-type bacteriaGram-positive bacterial pathogensPathogenic Gram-positive bacteriaStaphylococcus aureus cell wallBacterial cell wallBacterial cell surfaceEnzyme sortase ASmall moleculesGram-positive bacteriaWall peptidoglycanAureus cell wallMolecular handleBiosynthetic strategiesCell surfaceBacterial pathogensEndogenous levelsSortase AAzide-alkyne cycloaddition reactionChemical handlesWall engineeringBacteriaWidespread utility