2012
Identification of Core DNA Elements That Target Somatic Hypermutation
Kohler KM, McDonald JJ, Duke JL, Arakawa H, Tan S, Kleinstein SH, Buerstedde JM, Schatz DG. Identification of Core DNA Elements That Target Somatic Hypermutation. The Journal Of Immunology 2012, 189: 5314-5326. PMID: 23087403, PMCID: PMC3664039, DOI: 10.4049/jimmunol.1202082.Peer-Reviewed Original ResearchMeSH Keywords3' Flanking RegionAnimalsB-LymphocytesCells, CulturedChickensChromatin ImmunoprecipitationCytidine DeaminaseDNAEnhancer Elements, GeneticGenes, ImmunoglobulinGenetic LociImmunoassayImmunoglobulin Variable RegionMutationPhosphorylationRNA Polymerase IISerineSomatic Hypermutation, ImmunoglobulinTranscription, GeneticConceptsActivation-induced deaminaseDNA elementsSomatic hypermutationChicken DT40 B cellsIg lociChromatin immunoprecipitation experimentsDT40 B cellsRNA polymerase IISystematic deletion analysisL chain lociNon-Ig genesCore DNA elementSerine 5Epigenetic marksPolymerase IITranscriptional elongationMutational machineryDeletion analysisReporter cassetteImmunoprecipitation experimentsDeoxycytosine residuesIg genesDNA damageChain locusLoci
1997
A Basic Motif in the N-Terminal Region of RAG1 Enhances V(D)J Recombination Activity
McMahan C, Difilippantonio M, Rao N, Spanopoulou E, Schatz D. A Basic Motif in the N-Terminal Region of RAG1 Enhances V(D)J Recombination Activity. Molecular And Cellular Biology 1997, 17: 4544-4552. PMID: 9234712, PMCID: PMC232308, DOI: 10.1128/mcb.17.8.4544.Peer-Reviewed Original ResearchConceptsBasic amino acid motifN-terminal regionSite-specific DNA recognitionRecombination activityCell-specific componentsComponent gene segmentsExtrachromosomal recombination substratesAmino acid motifsAmino acids 216Site-specific recombination reactionAntigen receptor genesEvolutionary conservationNuclear transportRAG2 proteinsRAG1 proteinRecombination functionsRecombination substratesDeletion analysisDNA recognitionEnzymatic machineryAcid motifFurther mutagenesisSRP-1N-terminusRAG1