2003
Regulation of RAG1/RAG2‐mediated transposition by GTP and the C‐terminal region of RAG2
Tsai C, Schatz DG. Regulation of RAG1/RAG2‐mediated transposition by GTP and the C‐terminal region of RAG2. The EMBO Journal 2003, 22: 1922-1930. PMID: 12682024, PMCID: PMC154477, DOI: 10.1093/emboj/cdg185.Peer-Reviewed Original ResearchConceptsFull-length RAG2RAG2 proteinsRegulatory mechanismsC-terminal regionRAG proteinsHybrid joint formationDNA recognitionDNA transpositionCleavage functionChromosomal translocationsGTPUnknown mechanismRAG2ProteinTarget DNAPhysiological concentrationsRegulationJoint formationRAGRAG1MechanismTranslocationDNAGuanineTransposition
2000
The RAG Proteins and V(D)J Recombination: Complexes, Ends, and Transposition
Fugmann S, Lee A, Shockett P, Villey I, Schatz D. The RAG Proteins and V(D)J Recombination: Complexes, Ends, and Transposition. Annual Review Of Immunology 2000, 18: 495-527. PMID: 10837067, DOI: 10.1146/annurev.immunol.18.1.495.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1998
Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
Agrawal A, Eastman Q, Schatz D. Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system. Nature 1998, 394: 744-751. PMID: 9723614, DOI: 10.1038/29457.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesBinding SitesB-LymphocytesCatalysisCell LineDNADNA Transposable ElementsDNA, CircularDNA-Binding ProteinsDrug Resistance, MicrobialEvolution, MolecularGene Rearrangement, B-LymphocyteGene Rearrangement, T-LymphocyteHigh Mobility Group ProteinsHomeodomain ProteinsImmune SystemMiceMolecular Sequence DataReceptors, Antigen, T-CellRecombination, GeneticRestriction MappingTransposasesVertebratesConceptsT-cell receptor genesRecombination signalsSequence-specific DNA recognitionAncestral receptor geneComponent gene segmentsSite-specific recombination reactionPiece of DNAEvolutionary divergenceJawless vertebratesRecombination-activating geneTransposable elementsDNA recognitionRetroviral integrationGermline insertionDNA moleculesGenesShort duplicationsDNA cleavageRAG1Gene segmentsTransposition reactionRAG2Receptor geneTarget DNA moleculesTarget DNA
1997
A Basic Motif in the N-Terminal Region of RAG1 Enhances V(D)J Recombination Activity
McMahan C, Difilippantonio M, Rao N, Spanopoulou E, Schatz D. A Basic Motif in the N-Terminal Region of RAG1 Enhances V(D)J Recombination Activity. Molecular And Cellular Biology 1997, 17: 4544-4552. PMID: 9234712, PMCID: PMC232308, DOI: 10.1128/mcb.17.8.4544.Peer-Reviewed Original ResearchConceptsBasic amino acid motifN-terminal regionSite-specific DNA recognitionRecombination activityCell-specific componentsComponent gene segmentsExtrachromosomal recombination substratesAmino acid motifsAmino acids 216Site-specific recombination reactionAntigen receptor genesEvolutionary conservationNuclear transportRAG2 proteinsRAG1 proteinRecombination functionsRecombination substratesDeletion analysisDNA recognitionEnzymatic machineryAcid motifFurther mutagenesisSRP-1N-terminusRAG1