Measurement of estrogen receptors in intact cells by flow cytometry
Cao S, Hudnall S, Kohen F, Lu L. Measurement of estrogen receptors in intact cells by flow cytometry. Cytometry 2000, 41: 109-114. PMID: 11002266, DOI: 10.1002/1097-0320(20001001)41:2<109::aid-cyto5>3.0.co;2-7.Peer-Reviewed Original ResearchConceptsAnti-ER antibodiesDirect immunofluorescenceFlow cytometryIndirect immunofluorescenceEstrogen receptor levelsBreast cancer patientsER-negative MDAOutcome of treatmentHuman breast cancer cells MCF-7Anti-idiotypic antibodiesBreast cancer cells MCF-7Cancer cells MCF-7Flow cytometric analysisER immunostainingCells MCF-7Cancer patientsReceptor levelsEstrogen receptorER antibodyIntracellular antigensCytometric analysisTreatment of cellsTumor cellsAntibody clonesAntibodies