2024
The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells
Liao X, Li W, Zhou H, Rajendran B, Li A, Ren J, Luan Y, Calderwood D, Turk B, Tang W, Liu Y, Wu D. The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells. Nature Communications 2024, 15: 603. PMID: 38242867, PMCID: PMC10798966, DOI: 10.1038/s41467-024-44885-0.Peer-Reviewed Original ResearchConceptsCD8+ T cellsT cellsCancer immunotherapyMouse CD8+ T cellsAnti-tumor immunityTumor growth inhibition abilityAnti-tumor effectsInhibition of neddylationCD8Effector functionsTCR stimulationIL2 signalingCentral signaling pathwaysCore signaling pathwaysEffector activityNegative regulatory mechanismsTranslational implicationsImmunotherapyGrowth inhibition abilityCytokine signalingTCRProteomic alterationsSignaling pathwayCancerCRISPR-based screens
2020
Scaffold association factor B (SAFB) is required for expression of prenyltransferases and RAS membrane association
Zhou M, Kuruvilla L, Shi X, Viviano S, Ahearn IM, Amendola CR, Su W, Badri S, Mahaffey J, Fehrenbacher N, Skok J, Schlessinger J, Turk BE, Calderwood DA, Philips MR. Scaffold association factor B (SAFB) is required for expression of prenyltransferases and RAS membrane association. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 31914-31922. PMID: 33257571, PMCID: PMC7749360, DOI: 10.1073/pnas.2005712117.Peer-Reviewed Original ResearchMeSH KeywordsAlkyl and Aryl TransferasesCell MembraneComputational BiologyCRISPR-Cas SystemsDatasets as TopicDimethylallyltranstransferaseGene Knockdown TechniquesHumansMatrix Attachment Region Binding ProteinsNeoplasmsNuclear Matrix-Associated ProteinsProtein PrenylationProtein SubunitsProto-Oncogene Proteins p21(ras)Receptors, EstrogenConceptsMembrane associationRAS membrane associationFarnesyltransferase inhibitorsPrenylation pathwayGenome-wide CRISPRGTP loadingAlternative prenylationMutant cellsNuclear proteinsKRAS membrane associationsRAS isoformsΑ-subunitGrowth inhibitionExpressionFactor BPathwayAnticancer therapyAlternative therapeutic strategiesPrenyltransferasesRASTherapeutic strategiesCRISPRFarnesyltransferaseMislocalizationPrenylation