Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a
Zhang J, Li Z, Guo C, Guan X, Avery L, Banach D, Liu C. Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a. Angewandte Chemie 2024, 136 DOI: 10.1002/ange.202403123.Peer-Reviewed Original ResearchProtospacer adjacent motifTrans-cleavage activityUniversal assayCRISPR-Cas12a systemSingle-stranded DNAClinical samplesRNA substratesAdjacent motifRNA targetsRNA sensingDNA targetsNucleic acid detectionCas12a assayTranscription stepDNARNAMolecular diagnosticsHPV-16 DNADNA activityNucleic acid testingAcid detectionAssayHIV RNAClinical utilityProtospacerIntrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a
Zhang J, Li Z, Guo C, Guan X, Avery L, Banach D, Liu C. Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a. Angewandte Chemie International Edition 2024, 63: e202403123. PMID: 38516796, PMCID: PMC11073899, DOI: 10.1002/anie.202403123.Peer-Reviewed Original ResearchProtospacer adjacent motifTrans-cleavage activityUniversal assayCRISPR-Cas12a systemSingle-stranded DNAClinical samplesRNA substratesAdjacent motifRNA targetsRNA sensingDNA targetsNucleic acid detectionCas12a assayTranscription stepDNARNAMolecular diagnosticsHPV-16 DNADNA activityNucleic acid testingAcid detectionAssayHIV RNAClinical utilityProtospacer