2012
Kisspeptin Inhibits High-Voltage Activated Ca2+ Channels in GnRH Neurons via Multiple Ca2+ Influx and Release Pathways
Zhang XB, Spergel DJ. Kisspeptin Inhibits High-Voltage Activated Ca2+ Channels in GnRH Neurons via Multiple Ca2+ Influx and Release Pathways. Neuroendocrinology 2012, 96: 68-80. PMID: 22343183, DOI: 10.1159/000335985.Peer-Reviewed Original ResearchConceptsGnRH neuronsGnRH-green fluorescent protein transgenic miceG protein-coupled receptor 54Phospholipase C inhibitor edelfosineWhole-cell patch-clamp recordingsGonadotropin-releasing hormone (GnRH) secretionCanonical transient receptor potential (TRPC) channelsTransient receptor potential channelsRyR agonist caffeinePatch-clamp recordingsChelation of intracellularVGCC activityReceptor 54Kisspeptin-10Hormone secretionPeptide 234KP-10Antagonist caffeineBrain slicesSubsequent fertilityTransgenic miceTRPC channelsNeuronsDependent inhibitionCytoplasmic free
2007
Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty
Spergel DJ. Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty. Endocrinology 2007, 148: 2383-2390. PMID: 17289846, PMCID: PMC3315592, DOI: 10.1210/en.2006-1693.Peer-Reviewed Original ResearchConceptsAdult GnRH neuronsSK channel expressionGnRH neuronsGnRH secretionSK currentPubertal increaseGnRH-green fluorescent protein transgenic miceChannel expressionSmall-conductance calcium-activated potassium channelsGonadotropin-releasing hormone (GnRH) neuronsSK channel blocker apaminCalcium-activated potassium channelsCytoplasmic freeChannel blocker apaminExcitatory synaptic inputsWhole-cell techniqueSK channel subtypesHormone neuronsBlocker apaminKisspeptin stimulationChannel blockersSK channelsBrain slicesChannel subtypesSynaptic inputs
2003
A Confocal Microscopic Study of Gonadotropin-Releasing Hormone (GnRH) Neuron Inputs to Dopaminergic Neurons Containing Estrogen Receptor α in the Arcuate Nucleus of GnRH-Green Fluorescent Protein Transgenic Mice
Mitchell V, Loyens A, Spergel DJ, Flactif M, Poulain P, Tramu G, Beauvillain JC. A Confocal Microscopic Study of Gonadotropin-Releasing Hormone (GnRH) Neuron Inputs to Dopaminergic Neurons Containing Estrogen Receptor α in the Arcuate Nucleus of GnRH-Green Fluorescent Protein Transgenic Mice. Neuroendocrinology 2003, 77: 198-207. PMID: 12673053, DOI: 10.1159/000069511.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArcuate Nucleus of HypothalamusBrain MappingDopamineEstrogen Receptor alphaFemaleGonadotropin-Releasing HormoneGreen Fluorescent ProteinsHypothalamusLuminescent ProteinsMiceMice, Inbred C57BLMice, TransgenicMicroscopy, ConfocalNerve NetNeural PathwaysNeuronsReceptors, EstrogenTyrosine 3-MonooxygenaseConceptsGnRH-green fluorescent protein transgenic miceTH-ir neuronsTIDA neuronsArcuate nucleusGnRH neuronsDopaminergic neuronsFemale miceTransgenic miceTyrosine hydroxylase-immunoreactive neuronsTH monoclonal antibodyTuberoinfundibular dopaminergic neuronsHydroxylase-immunoreactive neuronsRole of estradiolAdult female miceDouble immunohistochemical stainingEstrogen receptor αGonadotropin-releasing hormone pathwayERalpha activationGnRH fibersImmunoreactive neuronsImmunohistochemical stainingBrain sectionsConfocal microscopic studiesReceptor αEstrus stage