2018
Pericyte ALK5/TIMP3 Axis Contributes to Endothelial Morphogenesis in the Developing Brain
Dave JM, Mirabella T, Weatherbee SD, Greif DM. Pericyte ALK5/TIMP3 Axis Contributes to Endothelial Morphogenesis in the Developing Brain. Developmental Cell 2018, 44: 665-678.e6. PMID: 29456135, PMCID: PMC5871595, DOI: 10.1016/j.devcel.2018.01.018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood-Brain BarrierBrainEmbryo, MammalianEndothelium, VascularFemaleHumansIntracranial HemorrhagesMaleMiceMice, Inbred C57BLMice, KnockoutMorphogenesisPericytesProtein Serine-Threonine KinasesReceptor, Transforming Growth Factor-beta Type IReceptors, Transforming Growth Factor betaSignal TransductionTissue Inhibitor of Metalloproteinase-3ConceptsEndothelial morphogenesisReceptor-like kinasesBlood-brain barrierActivin receptor-like kinaseGerminal matrixGerminal matrix hemorrhage-intraventricular hemorrhageMurine embryosEC morphogenesisEndothelial cellsEC hyperproliferationMorphogenesisMatrix metalloproteinase-3Glial precursorsBasement membrane collagenMatrix metalloproteinase activityBBB developmentMutantsVascular stabilityALK5Brain pericytesAbnormal dilationSubstantial therapeutic potentialMetalloproteinase-3Membrane collagenTissue inhibitor
2004
Calmodulin phosphorylation and modulation of endothelial nitric oxide synthase catalysis
Greif DM, Sacks DB, Michel T. Calmodulin phosphorylation and modulation of endothelial nitric oxide synthase catalysis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 1165-1170. PMID: 14736917, PMCID: PMC337024, DOI: 10.1073/pnas.0306377101.Peer-Reviewed Original ResearchConceptsPhosphorylation of CaMBovine aortic endothelial cellsCaM phosphorylationCaM mutantsCalcium regulatory protein calmodulinBiosynthetic labelingRegulation of eNOSProtein kinase pathwayEndothelial NO synthaseSer-81Regulatory protein calmodulinKinase CK2Kinase pathwayEndothelial cellsCK2Protein calmodulinMutantsCalmodulin phosphorylationPhosphorylationAortic endothelial cellsPhenyl-SepharoseUnique pathwayENOS activationMutant CaMPathway