2000
E2F-Rb Complexes Assemble and Inhibit cdc25A Transcription in Cervical Carcinoma Cells following Repression of Human Papillomavirus Oncogene Expression
Wu L, Goodwin E, Naeger L, Vigo E, Galaktionov K, Helin K, DiMaio D. E2F-Rb Complexes Assemble and Inhibit cdc25A Transcription in Cervical Carcinoma Cells following Repression of Human Papillomavirus Oncogene Expression. Molecular And Cellular Biology 2000, 20: 7059-7067. PMID: 10982822, PMCID: PMC86242, DOI: 10.1128/mcb.20.19.7059-7067.2000.Peer-Reviewed Original ResearchMeSH KeywordsBinding SitesBovine papillomavirus 1Carcinoma, Squamous CellCarrier ProteinsCdc25 PhosphatasesCell CycleCell Cycle ProteinsCell Transformation, NeoplasticCell Transformation, ViralConsensus SequenceCysteine EndopeptidasesDNA, NeoplasmDNA-Binding ProteinsE2F Transcription FactorsE2F4 Transcription FactorFemaleGene Expression Regulation, NeoplasticGene Expression Regulation, ViralGenes, RetinoblastomaHumansMacromolecular SubstancesMultienzyme ComplexesNeoplasm ProteinsPapillomaviridaePapillomavirus InfectionsPhosphoproteinsPromoter Regions, GeneticProteasome Endopeptidase ComplexProtein BindingProteinsRecombinant Fusion ProteinsRetinoblastoma ProteinRetinoblastoma-Binding Protein 1Retinoblastoma-Like Protein p130Transcription Factor DP1Transcription FactorsTransfectionTumor Cells, CulturedTumor Virus InfectionsUterine Cervical NeoplasmsViral ProteinsConceptsCdc25A promoterE6/E7 repressionCervical carcinoma cellsE2F siteBovine papillomavirus E2 proteinE2 proteinE7 repressionWild-type E2 proteinE2F-responsive promotersRb tumor suppressor pathwayPapillomavirus E2 proteinCarcinoma cellsE2F-Rb complexesCell cycle genesHuman papillomavirus oncogene expressionHuman papillomavirus (HPV) E6/E7 oncogenesTumor suppressor pathwayMechanism of repressionHPV E6/E7 expressionCell cycle progressionCdc25A transcriptionDramatic growth arrestE2F complexesConsensus E2FProtein complexes
1998
Oncogenic activation of the PDGF β receptor by the transmembrane domain of p185neu*
Petti L, Irusta P, DiMaio D. Oncogenic activation of the PDGF β receptor by the transmembrane domain of p185neu*. Oncogene 1998, 16: 843-851. PMID: 9484775, DOI: 10.1038/sj.onc.1201590.Peer-Reviewed Original ResearchConceptsBa/F3 cellsTransmembrane domainBa/F3 hematopoietic cellsF3 cellsWild-type PDGF receptorNovel tyrosine phosphorylated proteinsIL-3-independent growthTyrosine phosphorylated proteinsDistinct signaling pathwaysWild-type PDGFLevels of phosphotyrosineWild-type receptorIL-3Β receptorPDGF β-receptorPhosphorylated proteinsTyrosine autophosphorylationOncogenic formsKinase activityMouse C127Receptor homodimerizationOncogenic activationSignaling pathwaysChimeric receptorsFoci formation
1993
Platelet-derived growth factor receptor can mediate tumorigenic transformation by the bovine papillomavirus E5 protein.
Nilson L, DiMaio D. Platelet-derived growth factor receptor can mediate tumorigenic transformation by the bovine papillomavirus E5 protein. Molecular And Cellular Biology 1993, 13: 4137-4145. PMID: 8321218, PMCID: PMC359963, DOI: 10.1128/mcb.13.7.4137.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell Transformation, NeoplasticCell Transformation, ViralFemaleMiceMice, Inbred BALB CMice, NudeOncogene Proteins, ViralPrecipitin TestsReceptors, Platelet-Derived Growth FactorConceptsE5 proteinPDGF receptorPlatelet-derived growth factorBovine papillomavirus E5 proteinTumorigenic transformationMouse mammary gland cellsMurine mammary epithelial cell lineBovine papillomavirus type 1 E5 proteinPDGF receptor genesBPV E5 proteinMammary epithelial cell lineSustained proliferative signalEpidermal growth factor receptor (EGFR) pathwayPlatelet-derived growth factor receptorMammary gland cellsStable complexesGrowth factor receptor pathwayPDGF beta receptorTransforming proteinNMuMG cellsCellular proteinsGrowth factor receptorTyrosine phosphorylationEpithelial cell lineFibroblast transformation
1992
The central hydrophobic domain of the bovine papillomavirus E5 transforming protein can be functionally replaced by many hydrophobic amino acid sequences containing a glutamine
Kulke R, Horwitz B, Zibello T, DiMaio D. The central hydrophobic domain of the bovine papillomavirus E5 transforming protein can be functionally replaced by many hydrophobic amino acid sequences containing a glutamine. Journal Of Virology 1992, 66: 505-511. PMID: 1727496, PMCID: PMC238311, DOI: 10.1128/jvi.66.1.505-511.1992.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCell LineCell Transformation, NeoplasticCell Transformation, ViralDNAGlutamineMolecular Sequence DataOncogene Proteins, ViralPrecipitin TestsConceptsHydrophobic amino acidsAmino acidsE5 proteinRandom hydrophobic sequencesHydrophobic domainRodent fibroblast cell linesCentral hydrophobic domainHydrophobic amino acid sequenceCarboxyl-terminal amino acidsMouse C127 cellsAmino acid sequenceClasses of mutantsAbsence of glutamineBovine papillomavirus E5Mutant proteinsTransforming proteinDefective mutantsHydrophobic sequenceFibroblast cell lineProtein stabilityAcid sequenceC127 cellsHomodimer formationEfficient transformationProtein
1991
Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16
Leptak C, Cajal S, Kulke R, Horwitz B, Riese D, Dotto G, DiMaio D. Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16. Journal Of Virology 1991, 65: 7078-7083. PMID: 1658398, PMCID: PMC250837, DOI: 10.1128/jvi.65.12.7078-7083.1991.Peer-Reviewed Original ResearchConceptsBovine papillomavirus type 1E5 genePapillomavirus type 1Human Papillomavirus Type 16 E5 GeneTumorigenic transformationHost epithelial cellsExpression vectorRecombinant virusesRetroviral expression vectorMurine keratinocytesBiological propertiesTumorigenic cellsMurine epidermal keratinocytesGenesMurine fibroblastsFrameshift mutationCultured linesHuman papillomavirus type 16Cell linesEpithelial cellsPapillomavirus type 16Epidermal keratinocytesRetrovirusesCellsKeratinocytesActivation of the platelet‐derived growth factor receptor by the bovine papillomavirus E5 transforming protein.
Petti L, Nilson L, DiMaio D. Activation of the platelet‐derived growth factor receptor by the bovine papillomavirus E5 transforming protein. The EMBO Journal 1991, 10: 845-855. PMID: 1849073, PMCID: PMC452725, DOI: 10.1002/j.1460-2075.1991.tb08017.x.Peer-Reviewed Original ResearchConceptsPlatelet-derived growth factorE5 proteinBovine papillomavirus E5 proteinImportant cellular intermediatesPDGF receptorRodent fibroblast cell linesV-sis geneMembrane-associated proteinsStable growth transformationBovine papillomavirus E5Platelet-derived growth factor receptorSequence similarityCellular proteinsFibroblast cell lineGrowth factor receptorC127 cellsTumorigenic transformationE5 geneGrowth regulationCellular intermediatesFR3T3 cellsMature formShort regionGenetic studiesBeta-type receptorsTransforming Activity of Bovine and Human Papillomaviruses in Cultured Cells
DiMaio D. Transforming Activity of Bovine and Human Papillomaviruses in Cultured Cells. Advances In Cancer Research 1991, 56: 133-159. PMID: 1851373, DOI: 10.1016/s0065-230x(08)60480-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBovine papillomavirus 1CattleCell Transformation, NeoplasticCell Transformation, ViralCells, CulturedHumansPapillomaviridaeConceptsBovine papillomavirus type ICultured cellsTransient expression systemViral gene productsGene productsComplex phenotypesExpression systemTumorigenic transformationBiochemical eventsMolecular descriptionCell morphologyCottontail rabbit papillomavirusBiological responsesHuman carcinomasCultured fibroblastsGrowth propertiesCellsRabbit papillomavirusHomogeneous populationProfound effectGenesType ISimilar activityTumorigenesisPhenotype
1990
Bovine papillomavirus E2 repressor mutant displays a high-copy-number phenotype and enhanced transforming activity
Riese D, Settleman J, Neary K, DiMaio D. Bovine papillomavirus E2 repressor mutant displays a high-copy-number phenotype and enhanced transforming activity. Journal Of Virology 1990, 64: 944-949. PMID: 2153255, PMCID: PMC249196, DOI: 10.1128/jvi.64.2.944-949.1990.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBovine papillomavirus 1Cell LineCell Transformation, NeoplasticDNA, ViralGenes, RegulatorGenes, ViralMutationOligonucleotide ProbesPapillomaviridaePhenotypeTranscriptional ActivationConceptsRepressor proteinWild-type copy numberBovine papillomavirus type 1 genomeCopy numberViral genomeTranscriptional repressor proteinTransient expression experimentsMouse C127 cellsMutant viral DNAViral DNAType 1 genomeNumber phenotypeRepressor activityLow copy numberMethionine codonInitiation codonExpression experimentsC127 cellsGenomeCV1 cellsRepressor mutantsFoci formationColony formationCodonProtein
1989
Genetic evidence that acute morphologic transformation, induction of cellular DNA synthesis, and focus formation are mediated by a single activity of the bovine papillomavirus E5 protein.
Settleman J, Fazeli A, Malicki J, Horwitz B, DiMaio D. Genetic evidence that acute morphologic transformation, induction of cellular DNA synthesis, and focus formation are mediated by a single activity of the bovine papillomavirus E5 protein. Molecular And Cellular Biology 1989, 9: 5563-5572. PMID: 2555701, PMCID: PMC363726, DOI: 10.1128/mcb.9.12.5563.Peer-Reviewed Original ResearchConceptsE5 proteinE5 geneCellular DNA synthesisC127 cellsBovine papillomavirus E5 proteinMouse C127 cellsDNA synthesisMorphologic transformationCultured rodent cellsDefective phenotypeMissense mutantsUnstable proteinDefective mutantsGenetic evidenceMutational analysisE5 activityRodent cellsCell cycleViral genesBiochemical activitySerum starvationCell transformationGenesContact inhibitionVirus multiplicityTransforming activity of a 16-amino-acid segment of the bovine papillomavirus E5 protein linked to random sequences of hydrophobic amino acids
Horwitz B, Weinstat D, DiMaio D. Transforming activity of a 16-amino-acid segment of the bovine papillomavirus E5 protein linked to random sequences of hydrophobic amino acids. Journal Of Virology 1989, 63: 4515-4519. PMID: 2552136, PMCID: PMC251082, DOI: 10.1128/jvi.63.11.4515-4519.1989.Peer-Reviewed Original ResearchConceptsE5 proteinAmino acidsWild-type E5 proteinBovine papillomavirus E5 proteinAmino acid sequence requirementsHydrophobic amino acid sequenceCarboxyl-terminal amino acidsMouse C127 cellsAmino acid sequenceBovine papillomavirus type 1Carboxyl-terminal portionWild-type onesHydrophobic amino acidsPapillomavirus type 1Hydrophobic sequenceDifferent amino acidsAcid sequenceC127 cellsSequence requirementsE5 geneCell transformationFoci formationSubstitution mutationsCell membraneProteinBovine Papillomavirus Type I Induces Resistance to Ca++-Induced Terminal Differentiation in Murine Keratinocytes
Reiss M, DiMaio D, Zibello T. Bovine Papillomavirus Type I Induces Resistance to Ca++-Induced Terminal Differentiation in Murine Keratinocytes. Oncology Research Featuring Preclinical And Clinical Cancer Therapeutics 1989, 1: 75-82. PMID: 2561735, DOI: 10.3727/095535489820875318.Peer-Reviewed Original Research
1988
Efficient transactivation and morphologic transformation by bovine papillomavirus genes expressed from a bovine papillomavirus/simian virus 40 recombinant virus.
Settleman J, DiMaio D. Efficient transactivation and morphologic transformation by bovine papillomavirus genes expressed from a bovine papillomavirus/simian virus 40 recombinant virus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 9007-9011. PMID: 2848252, PMCID: PMC282651, DOI: 10.1073/pnas.85.23.9007.Peer-Reviewed Original ResearchMeSH KeywordsBeta-GalactosidaseBovine papillomavirus 1Cell LineCell Transformation, NeoplasticGene Expression RegulationGenesGenes, ViralHybridization, GeneticPapillomaviridaePlasmidsSimian virus 40Viral ProteinsConceptsBovine papillomavirus type 1 E2Simian virus 40 early regionTransactivation of genesCultured mammalian cellsHybrid viral genomesSimian virus 40 virionsMouse C127 cellsSimian virus 40 large tumor antigenBovine papillomavirus type 1Large tumor antigenPapillomavirus type 1E5 mutantsAcute cellular responseRegulatory genesMammalian cellsControl regionEfficient transactivationForeign genesReporter geneType 1 geneWild-type virusC127 cellsE5 geneTumorigenic transformationCellular responses
1987
Mutational analysis of open reading frame E4 of bovine papillomavirus type 1
Neary K, Horwitz B, DiMaio D. Mutational analysis of open reading frame E4 of bovine papillomavirus type 1. Journal Of Virology 1987, 61: 1248-1252. PMID: 3029420, PMCID: PMC254088, DOI: 10.1128/jvi.61.4.1248-1252.1987.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBovine papillomavirus 1CattleCell LineCell Transformation, NeoplasticDNA Restriction EnzymesGenes, ViralMiceMutationPapillomaviridaePlasmidsConceptsBovine papillomavirus type 1Papillomavirus type 1E4 proteinLate gene expressionMouse C127 cellsAmino acid sequenceAcid sequenceExtrachromosomal plasmidsBiological activityC127 cellsMutational analysisGene expressionFoci formationORF E2ORFProteinViral DNAMutationsSoft agaroseCellsMutantsType 1Papilloma formationDNAPlasmid