Heat‐shock–mediated conditional regulation of hedgehog/gli signaling in zebrafish
Shen M, Ozacar A, Osgood M, Boeras C, Pink J, Thomas J, Kohtz J, Karlstrom R. Heat‐shock–mediated conditional regulation of hedgehog/gli signaling in zebrafish. Developmental Dynamics 2013, 242: 539-549. PMID: 23441066, DOI: 10.1002/dvdy.23955.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedEmbryo, NonmammalianGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsHeat-Shock ResponseHedgehog ProteinsHSP70 Heat-Shock ProteinsOncogene ProteinsPromoter Regions, GeneticSignal TransductionTrans-ActivatorsZebrafishZebrafish ProteinsZinc Finger Protein GLI1ConceptsTransgenic linesConditional regulationHeat shockEmbryonic central nervous systemZebrafish transgenic linesNew reporter linesHeat shock activationInducible transgenic linesHedgehog/GLIStem cell proliferationSingle heat shockHh responseHh functionFunction phenotypesPostembryonic rolesReporter linesPromoter elementsHedgehog signalingJuvenile stageSitu hybridizationCell proliferationEarly embryonic lossQuantitative polymerase chain reactionLife cycleNew experimental tools