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Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nature Biotechnology 2016, 34: 184-191. PMID: 26780180, PMCID: PMC4744125, DOI: 10.1038/nbt.3437.
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