Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophages
Roy C, Isberg R. Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophages. Infection And Immunity 1997, 65: 571-578. PMID: 9009315, PMCID: PMC176098, DOI: 10.1128/iai.65.2.571-578.1997.Peer-Reviewed Original ResearchConceptsDotA mutantsAmino acidsMembrane proteinsIntegral cytoplasmic membrane proteinLarge periplasmic domainInner membrane proteinCarboxyl-terminal cytoplasmic domainIntracellular growthCytoplasmic membrane proteinAlkaline phosphatase fusionsL. pneumophila pathogenesisIntracellular growth defectMembrane-spanning domainsStructure-function analysisProtein fractionation studiesPeriplasmic domainDotA proteinTranslational fusionGrowth defectCytoplasmic domainInner membraneHybrid proteinCytoplasmic regionDotA geneC-terminus