2000
Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth
Coers J, Kagan J, Matthews M, Nagai H, Zuckman D, Roy C. Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth. Molecular Microbiology 2000, 38: 719-736. PMID: 11115108, DOI: 10.1046/j.1365-2958.2000.02176.x.Peer-Reviewed Original ResearchConceptsPhagosome traffickingTransport apparatusHost cellsProtein-protein interactionsSubset of genesGel overlay analysisDistinct phenotypic categoriesPhagocytic host cellsIcm genesTranslocation channelReplicative organelleTwo-hybridProtein complexesSpecialized organellesTransporter functionIntracellular growthMolecular levelDistinct rolesVirulence determinantsGenesPore formationBacterial pathogensBiogenesisPhenotypic categoriesLegionella pneumophila
1999
Pore‐forming activity is not sufficient for Legionella pneumophila phagosome trafficking and intracellular growth
Zuckman D, Hung J, Roy C. Pore‐forming activity is not sufficient for Legionella pneumophila phagosome trafficking and intracellular growth. Molecular Microbiology 1999, 32: 990-1001. PMID: 10361301, DOI: 10.1046/j.1365-2958.1999.01410.x.Peer-Reviewed Original ResearchConceptsPhagosome traffickingPhagosome-lysosome fusionIntracellular growthLysosome fusionEukaryotic cellular processesInsertion of poresPore-forming activityL. pneumophila mutantsHost cell cytoplasmCellular processesMammalian cellsReplicative nicheSimilar cytolytic activityGene productsPhagosome membraneIntracellular bacteriaTraffickingCell cytoplasmEffector moleculesBacterial pathogensLegionella pneumophilaMacrophage membraneVirulent bacteriaBacteriaFusion inhibition
1997
Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophages
Roy C, Isberg R. Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophages. Infection And Immunity 1997, 65: 571-578. PMID: 9009315, PMCID: PMC176098, DOI: 10.1128/iai.65.2.571-578.1997.Peer-Reviewed Original ResearchConceptsDotA mutantsAmino acidsMembrane proteinsIntegral cytoplasmic membrane proteinLarge periplasmic domainInner membrane proteinCarboxyl-terminal cytoplasmic domainIntracellular growthCytoplasmic membrane proteinAlkaline phosphatase fusionsL. pneumophila pathogenesisIntracellular growth defectMembrane-spanning domainsStructure-function analysisProtein fractionation studiesPeriplasmic domainDotA proteinTranslational fusionGrowth defectCytoplasmic domainInner membraneHybrid proteinCytoplasmic regionDotA geneC-terminus