2000
Identification and Subcellular Localization of the Legionella pneumophila IcmX Protein: a Factor Essential for Establishment of a Replicative Organelle in Eukaryotic Host Cells
Matthews M, Roy C. Identification and Subcellular Localization of the Legionella pneumophila IcmX Protein: a Factor Essential for Establishment of a Replicative Organelle in Eukaryotic Host Cells. Infection And Immunity 2000, 68: 3971-3982. PMID: 10858211, PMCID: PMC101675, DOI: 10.1128/iai.68.7.3971-3982.2000.Peer-Reviewed Original ResearchConceptsEukaryotic host cellsReplicative organelleGene productsHost cellsSecretion apparatusDot/Icm proteinsDot/icm genesWild-type L. pneumophilaL. pneumophila chromosomePathogen Legionella pneumophilaHost cell parasitismImmunoblot analysisMurine bone marrow-derived macrophagesL. pneumophilaConjugal transfer systemObvious orthologsBone marrow-derived macrophagesIcm genesBacterial periplasmCell parasitismMammalian macrophagesDeletion mutantsSubcellular localizationPhagosome biogenesisMarrow-derived macrophages
1999
Pore‐forming activity is not sufficient for Legionella pneumophila phagosome trafficking and intracellular growth
Zuckman D, Hung J, Roy C. Pore‐forming activity is not sufficient for Legionella pneumophila phagosome trafficking and intracellular growth. Molecular Microbiology 1999, 32: 990-1001. PMID: 10361301, DOI: 10.1046/j.1365-2958.1999.01410.x.Peer-Reviewed Original ResearchConceptsPhagosome traffickingPhagosome-lysosome fusionIntracellular growthLysosome fusionEukaryotic cellular processesInsertion of poresPore-forming activityL. pneumophila mutantsHost cell cytoplasmCellular processesMammalian cellsReplicative nicheSimilar cytolytic activityGene productsPhagosome membraneIntracellular bacteriaTraffickingCell cytoplasmEffector moleculesBacterial pathogensLegionella pneumophilaMacrophage membraneVirulent bacteriaBacteriaFusion inhibition