Featured Publications
A Screen of Coxiella burnetii Mutants Reveals Important Roles for Dot/Icm Effectors and Host Autophagy in Vacuole Biogenesis
Newton HJ, Kohler LJ, McDonough JA, Temoche-Diaz M, Crabill E, Hartland EL, Roy CR. A Screen of Coxiella burnetii Mutants Reveals Important Roles for Dot/Icm Effectors and Host Autophagy in Vacuole Biogenesis. PLOS Pathogens 2014, 10: e1004286. PMID: 25080348, PMCID: PMC4117601, DOI: 10.1371/journal.ppat.1004286.Peer-Reviewed Original ResearchConceptsHost autophagyTransposon insertionDot/Icm effectorsDot/Icm systemAutophagosome protein LC3Intracellular growth defectPathogen-occupied vacuolesTransposon insertion mutantsVacuole biogenesisEffector proteinsInsertion mutantsModification enzymesGrowth defectArrayed libraryCentral metabolismIcm systemMutantsMolecular mechanismsVisual screenProtein LC3Host cellsIntracellular replicationGenesIntracellular pathogensRegulatory systemThe Coxiella burnetii effector EmcB is a deubiquitinase that inhibits RIG-I signaling
Duncan-Lowey J, Crabill E, Jarret A, Reed S, Roy C. The Coxiella burnetii effector EmcB is a deubiquitinase that inhibits RIG-I signaling. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2217602120. PMID: 36893270, PMCID: PMC10089202, DOI: 10.1073/pnas.2217602120.Peer-Reviewed Original ResearchConceptsDot/Icm systemHost-adapted pathogenUbiquitin chainsI interferon productionType I interferon productionSecretion systemHost cytosolIcm systemInterferon productionProtein secretion systemBacterial secretion systemsAcid-inducible gene IHost surveillance systemImmune sensorsObligate intracellular pathogensInducible gene ICytosolic surveillance systemsProtective immune responseEffector proteinsSpecialized organellesVacuolar nicheInhibition of RIGInnate immune sensorsSurveillance systemMammalian hosts
2024
InSeq analysis of defined Legionella pneumophila libraries identifies a transporter-encoding gene cluster important for intracellular replication in mammalian hosts.
Moss C, Roy C. InSeq analysis of defined Legionella pneumophila libraries identifies a transporter-encoding gene cluster important for intracellular replication in mammalian hosts. MBio 2024, e0195524. PMID: 39365064, DOI: 10.1128/mbio.01955-24.Peer-Reviewed Original ResearchMammalian hostsLegionella pneumophila</i>Mutant libraryIntracellular replicationDot/Icm type IV secretion systemHost cellsType IV secretion systemInfection of mammalian cellsTransposon insertion sequencingTransposon mutant libraryOpportunistic human pathogenIV secretion systemIsogenic deletion mutantsGenetic complementation studiesHost-pathogen interactionsIntracellular bacterial pathogensMultidrug efflux pumpsMammalian infection modelsATP-binding cassetteBacteria encounterDot/Icm systemSecretion systemTransposon sequencesGene clusterHost cytosol