1999
Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome
Peterson M, Burd C, Emr S. Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome. Current Biology 1999, 9: 159-s1. PMID: 10021387, DOI: 10.1016/s0960-9822(99)80071-2.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingCytoskeletal ProteinsEndosomesFungal ProteinsGolgi ApparatusGTP PhosphohydrolasesGTP-Binding ProteinsGuanine Nucleotide Dissociation InhibitorsGuanosine TriphosphateMacromolecular SubstancesMembrane ProteinsPhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesProtein BindingQa-SNARE ProteinsRab GTP-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsVesicular Transport ProteinsConceptsVacuolar protein sorting (VPS) pathwayVesicle docking/fusionProtein sorting pathwaysDocking/fusionVacuolar protein precursorsMultivalent adaptor proteinLysosome-like vacuolePrevacuolar endosomeVPS genesVps34 phosphatidylinositolLate GolgiGTPase signalsRab GTPaseSorting pathwaysVesicle dockingEndosome transportVacuolar proteinAdaptor proteinEndosomal compartmentsVac1pProtein precursorGolgiTransport stepsVps21pVps45p
1998
Acidic Di-leucine Motif Essential for AP-3–dependent Sorting and Restriction of the Functional Specificity of the Vam3p Vacuolar t-SNARE
Darsow T, Burd C, Emr S. Acidic Di-leucine Motif Essential for AP-3–dependent Sorting and Restriction of the Functional Specificity of the Vam3p Vacuolar t-SNARE. Journal Of Cell Biology 1998, 142: 913-922. PMID: 9722605, PMCID: PMC2132875, DOI: 10.1083/jcb.142.4.913.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Vesicular TransportAlkaline PhosphataseBiological TransportCarboxypeptidasesCathepsin AEndosomesFungal ProteinsLeucineMembrane ProteinsMonomeric Clathrin Assembly ProteinsMutationNerve Tissue ProteinsPhosphoproteinsQa-SNARE ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSNARE ProteinsVesicle-Associated Membrane Protein 3Vesicular Transport ProteinsConceptsPre-vacuolar endosomesVacuolar proteinCarboxypeptidase Y pathwayDi-leucine sequenceOrganelle-specific functionsT-SNARE proteinsAP-3 functionAlkaline phosphatase pathwayYeast Saccharomyces cerevisiaeTransport of proteinsSNAP receptor (SNARE) proteinsT-SNAREsGenetic screenCPY pathwayLeucine signalDelivery of cargoPhosphatase pathwaysRegulated transportMammalian systemsPep12pVam3pSaccharomyces cerevisiaeAccessory proteinsAcid sequenceALP pathway
1997
A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast.
Burd C, Peterson M, Cowles C, Emr S. A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast. Molecular Biology Of The Cell 1997, 8: 1089-1104. PMID: 9201718, PMCID: PMC305716, DOI: 10.1091/mbc.8.6.1089.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAdenosine TriphosphatasesBiological TransportCell CompartmentationCytoskeletal ProteinsEndosomesFungal ProteinsGolgi ApparatusGTP-Binding ProteinsMacromolecular SubstancesMembrane FusionMembrane ProteinsProtein BindingQa-SNARE ProteinsRab GTP-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsStructure-Activity RelationshipVacuolesVesicular Transport ProteinsZincConceptsVacuolar protein sortingProtein sortingVPS pathwayEndosome transportSevere synthetic growth defectVacuolar protein sorting (VPS) genesVacuolar protein sorting (VPS) pathwaySynthetic growth defectsProtein sorting pathwaysZinc finger motifsRab family GTPaseCoiled-coil motifLocalization of proteinsSite-directed mutationsSDS-resistant complexesDominant negative mutationTsf phenotypeTsf mutationsFinger motifVesicle receptorsEndosome traffickingVacuole inheritanceVac1pVacuolar proteinTrans-GolgiNovel Golgi to vacuole delivery pathway in yeast: identification of a sorting determinant and required transport component
Cowles C, Snyder W, Burd C, Emr S. Novel Golgi to vacuole delivery pathway in yeast: identification of a sorting determinant and required transport component. The EMBO Journal 1997, 16: 2769-2782. PMID: 9184222, PMCID: PMC1169886, DOI: 10.1093/emboj/16.10.2769.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAmino Acid SequenceBiological TransportCarboxypeptidasesCarrier ProteinsCathepsin ACell CompartmentationFungal ProteinsGolgi ApparatusMembrane ProteinsModels, BiologicalMolecular Sequence DataNuclear ProteinsQa-SNARE ProteinsRecombinant Fusion ProteinsRNA-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsVacuolesVesicular Transport ProteinsConceptsVacuolar protein sorting (vps) mutantsSoluble vacuolar hydrolasesVacuolar membrane proteinSoluble vacuolar proteinsDomain-swapping experimentsNon-permissive conditionsNon-permissive temperatureCytoplasmic tail domainVacuolar deliveryT-SNAREsNovel GolgiTransport intermediatesSorting signalsVacuolar hydrolasesVesicle recognitionVacuolar proteinDouble mutantAmino acid portionVacuolar localizationMembrane proteinsTail domainDelivery pathwayPlasma membraneSelective packagingAlkaline phosphatase