2001
RNA interference in Trypanosoma brucei: cloning of small interfering RNAs provides evidence for retroposon-derived 24-26-nucleotide RNAs.
Djikeng A, Shi H, Tschudi C, Ullu E. RNA interference in Trypanosoma brucei: cloning of small interfering RNAs provides evidence for retroposon-derived 24-26-nucleotide RNAs. RNA 2001, 7: 1522-30. PMID: 11720282, PMCID: PMC1370195.Peer-Reviewed Original ResearchConceptsDouble-stranded RNARNA interferenceGene-specific double-stranded RNAHigh-speed pellet fractionStrand-specific probesSmall interfering RNAsTarget RNA degradationDsRNA resultsHousekeeping functionsTrypanosoma bruceiRNA degradationCellular RNAInterfering RNAsNorthern hybridizationWeight complexesSequence analysisHigh-speed pelletRNALong fragmentSiRNAsPellet fractionEnrichment strategyCloningSupernatant fractionFragments
1998
Trypanosome Capping Enzymes Display a Novel Two-Domain Structure
Silva E, Ullu E, Kobayashi R, Tschudi C. Trypanosome Capping Enzymes Display a Novel Two-Domain Structure. Molecular And Cellular Biology 1998, 18: 4612-4619. PMID: 9671471, PMCID: PMC109047, DOI: 10.1128/mcb.18.8.4612.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCloning, MolecularConserved SequenceCrithidia fasciculataDNA, ProtozoanGuanosine MonophosphateHumansMiceMolecular Sequence DataRecombinant Fusion ProteinsRNA NucleotidyltransferasesSequence Homology, Amino AcidTrypanosoma brucei bruceiConceptsSpliceosomal small nuclear RNAsM7G capCapping enzymeTwo-domain structureG capYeast capping enzymesSmall nuclear RNAPhosphate-binding loopAmino-terminal halfCarboxy-terminal halfGTP-binding proteinsDiphosphate endTrans splicingSignature motifTranscript elongationM7G residueSL sequenceTrypanosomatid protozoaEukaryotic mRNAsLeader RNAGene codesRNA modificationsNuclear RNAOverall identityTrypanosoma brucei
1990
Conserved sequences in the U2 snRNA-encoding genes of Kinetoplastida do not include the putative branchpoint recognition region
Tschudi C, Williams S, Ullu E. Conserved sequences in the U2 snRNA-encoding genes of Kinetoplastida do not include the putative branchpoint recognition region. Gene 1990, 91: 71-77. PMID: 2401409, DOI: 10.1016/0378-1119(90)90164-m.Peer-Reviewed Original ResearchConceptsU2 small nuclear RNASmall nuclear RNAU2 genesEvolutionary conservationOrder KinetoplastidaConserved sequencesNuclear RNASequence variationFlanking regionsNuclear extractsRecognition sequenceKinetoplastidaUpstream elementRecognition regionGenesRNACorresponding sequenceLeishmania mexicana amazonensisSequenceT. congolenseTrypanosoma congolenseBiosynthesisNucleotidesProteinCongolense
1988
Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes.
Tschudi C, Ullu E. Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes. The EMBO Journal 1988, 7: 455-463. PMID: 3366120, PMCID: PMC454342, DOI: 10.1002/j.1460-2075.1988.tb02833.x.Peer-Reviewed Original ResearchConceptsCalmodulin mRNACultured procyclic trypanosomesAbundant RNA speciesSteady-state RNAPulse-labeled RNAAuthentic precursorIntergenic regionRNA speciesProcyclic trypanosomesCalmodulin genePolyadenylation sitesAfrican trypanosomesHybridization probesTranscriptsStreptavidin-agaroseDiscrete endsSmall familyTrypanosomesMRNAGenesRNASpeciesCalmodulinTrypanosoma brucei gambienseBrucei gambiense
1986
The U2 RNA analogue of Trypanosoma brucei gambiense: implications for a splicing mechanism in trypanosomes
Tschudi C, Richards F, Ullu E. The U2 RNA analogue of Trypanosoma brucei gambiense: implications for a splicing mechanism in trypanosomes. Nucleic Acids Research 1986, 14: 8893-8903. PMID: 3786140, PMCID: PMC311918, DOI: 10.1093/nar/14.22.8893.Peer-Reviewed Original ResearchConceptsU2 RNASpliced leader RNASingle-copy geneSignificant sequence homologyMaturation of mRNAMammalian counterpartsCopy geneU RNALeader RNASplicing mechanismSequence homologySecondary structureRNATrypanosomesGenesRNA analogSplicingHomologyTrypanosoma brucei gambiensePossible interactionsMRNABrucei gambienseMaturation
1985
Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.
Tschudi C, Young A, Ruben L, Patton C, Richards F. Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 3998-4002. PMID: 3858856, PMCID: PMC397921, DOI: 10.1073/pnas.82.12.3998.Peer-Reviewed Original Research
1983
Reverse transcription of trypanosome variable antigen mRNAs initiated by a specific oligonucleotide primer.
Merritt S, Tschudi C, Konigsberg W, Richards F. Reverse transcription of trypanosome variable antigen mRNAs initiated by a specific oligonucleotide primer. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 1536-1540. PMID: 6188156, PMCID: PMC393636, DOI: 10.1073/pnas.80.6.1536.Peer-Reviewed Original Research