2001
RNA interference in Trypanosoma brucei: cloning of small interfering RNAs provides evidence for retroposon-derived 24-26-nucleotide RNAs.
Djikeng A, Shi H, Tschudi C, Ullu E. RNA interference in Trypanosoma brucei: cloning of small interfering RNAs provides evidence for retroposon-derived 24-26-nucleotide RNAs. RNA 2001, 7: 1522-30. PMID: 11720282, PMCID: PMC1370195.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCloning, MolecularGene SilencingRetroelementsRNA Processing, Post-TranscriptionalRNA, Double-StrandedRNA, ProtozoanRNA, Small InterferingRNA, UntranslatedTrypanosoma brucei bruceiConceptsDouble-stranded RNARNA interferenceGene-specific double-stranded RNAHigh-speed pellet fractionStrand-specific probesSmall interfering RNAsTarget RNA degradationDsRNA resultsHousekeeping functionsTrypanosoma bruceiRNA degradationCellular RNAInterfering RNAsNorthern hybridizationWeight complexesSequence analysisHigh-speed pelletRNALong fragmentSiRNAsPellet fractionEnrichment strategyCloningSupernatant fractionFragments
1995
Accurate Modification of the Trypanosome Spliced Leader Cap Structure in a Homologous Cell-free System (∗)
Ullu E, Tschudi C. Accurate Modification of the Trypanosome Spliced Leader Cap Structure in a Homologous Cell-free System (∗). Journal Of Biological Chemistry 1995, 270: 20365-20369. PMID: 7657609, DOI: 10.1074/jbc.270.35.20365.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCell-Free SystemCrithidia fasciculataMethylationRNA CapsRNA Processing, Post-TranscriptionalRNA SplicingRNA, MessengerRNA, ProtozoanTrypanosoma brucei bruceiConceptsSL RNACap structureCell-free systemRibonucleoprotein particleUnusual cap structureSpliced leader sequenceTrans-splicing activityHomologous cell-free systemRNA maturationTrypanosomatid protozoaNucleotide compositionC. fasciculataLeader sequenceTrypanosoma bruceiCell-free extractsT. bruceiT. cruziRNANucleotides 7Vitro reactionBruceiTwo-dimensional thin-layer chromatographyL. amazonensisLeishmania amazonensisCrithidia
1991
Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA.
Ullu E, Tschudi C. Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1991, 88: 10074-10078. PMID: 1719544, PMCID: PMC52870, DOI: 10.1073/pnas.88.22.10074.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsMethylationNucleotide MappingRibonucleasesRNARNA Processing, Post-TranscriptionalRNA SplicingRNA, MessengerTranscription, GeneticTrypanosoma brucei bruceiTubulin
1985
Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.
Tschudi C, Young A, Ruben L, Patton C, Richards F. Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 3998-4002. PMID: 3858856, PMCID: PMC397921, DOI: 10.1073/pnas.82.12.3998.Peer-Reviewed Original Research