1998
Double-stranded RNA induces mRNA degradation in Trypanosoma brucei
Ngô H, Tschudi C, Gull K, Ullu E. Double-stranded RNA induces mRNA degradation in Trypanosoma brucei. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 14687-14692. PMID: 9843950, PMCID: PMC24510, DOI: 10.1073/pnas.95.25.14687.Peer-Reviewed Original ResearchConceptsMRNA degradationUntranslated regionFlagellar attachment zoneMRNA 5' untranslated regionAlpha-tubulin synthesisImportant protozoan parasitesCaenorhabditis elegansAlpha-tubulin mRNACellular mRNAsTrypanosome cellsGenetic interferenceCytoskeletal structuresCleavage furrowDsRNA transfectionSame phenotypeCytokinesisComplex cortical structureFlagellar axonemeTubulin mRNAPlasmid vectorProtozoan parasiteVivo expressionAttachment zoneMRNADsRNA
1995
Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *
Günzl A, Tschudi C, Nakaar V, Ullu E. Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *. Journal Of Biological Chemistry 1995, 270: 17287-17291. PMID: 7542235, DOI: 10.1074/jbc.270.29.17287.Peer-Reviewed Original ResearchConceptsIntragenic control regionRNA polymerase IIIControl regionPolymerase IIIU2 small nuclear RNA genesTranscription systemSmall nuclear RNA genesGene expressionInsect form trypanosomesNuclear RNA genesU2 snRNA genesSnRNA gene promotersB-box elementsFull transcriptional activitySeries of mutationsSnRNA genesRNA genesInsect formsRNA polymeraseGene promoterTrypanosoma bruceiCell-free extractsTransient expressionBox elementTranscriptional activity
1994
Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes.
Nakaar V, Dare A, Hong D, Ullu E, Tschudi C. Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes. Molecular And Cellular Biology 1994, 14: 6736-6742. PMID: 7523857, PMCID: PMC359204, DOI: 10.1128/mcb.14.10.6736.Peer-Reviewed Original ResearchConceptsSmall RNA genesTRNA genesRNA genesSmall nuclear RNARegulatory elementsU6 small nuclear RNAUpstream tRNA genesIntragenic promoter elementsT. brucei cellsU6 snRNA genesRNA polymerase IIIB-box elementsSnRNA lociGenome organizationSnRNA genesRNA lociSmall RNAsBrucei cellsB-boxSubstitution mutantsPolymerase IIIFamily TrypanosomatidaeNuclear RNAGene transcriptionPromoter elements
1991
Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA.
Ullu E, Tschudi C. Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1991, 88: 10074-10078. PMID: 1719544, PMCID: PMC52870, DOI: 10.1073/pnas.88.22.10074.Peer-Reviewed Original Research
1990
Destruction of U2, U4, or U6 small nuclear RNA blocks Trans splicing in trypanosome cells
Tschudi C, Ullu E. Destruction of U2, U4, or U6 small nuclear RNA blocks Trans splicing in trypanosome cells. Cell 1990, 61: 459-466. PMID: 1692263, DOI: 10.1016/0092-8674(90)90527-l.Peer-Reviewed Original ResearchConceptsTrans splicingU snRNAsPre-mRNAU6 small nuclear RNAU4/U6 snRNPsAlpha-tubulin transcriptsSmall nuclear RNAHigh molecular weight RNAMolecular weight RNASite-directed cleavageSL sequenceLeader RNATrypanosome cellsU6 snRNPsNuclear RNAU6 snRNATrypanosoma bruceiSplicing processSplicingWeight RNASnRNAEarly stepsRNAase HRNAIntrons
1984
Alu sequences are processed 7SL RNA genes
Ullu E, Tschudi C. Alu sequences are processed 7SL RNA genes. Nature 1984, 312: 171-172. PMID: 6209580, DOI: 10.1038/312171a0.Peer-Reviewed Original ResearchConceptsRNA genesAlu sequencesMiddle repetitive DNA sequencesAbundant cytoplasmic RNARepetitive DNA sequencesHuman 7SL RNAInsect sequencesAlu domainMammalian radiationDrosophila melanogasterEvolutionary distanceXenopus sequenceAbundant familyDNA sequencesNucleotide sequenceCDNA copyCytoplasmic RNAPair sequencePrimary structureProtein secretionRNAGenesHuman counterpartSequenceSignificant block